Nutritional composition, physicochemical, microbiological, and sensory properties of flavored biofermented camel milk containing chickpea milk
Main Article Content
Keywords
camel milk; chickpea milk; probiotic viability; sensory properties; date syrup
Abstract
Worldwide, the consumption of plant-based dairy alternatives has increased rapidly due to numerous positive health effects. There is very little information available about the potential use of chickpea seed milk in the manufacture of fermented camel milk (FCM). This study investigated the effect of partial replacement of camel milk (CM) with chickpea milk (ChM) at different concentrations (0%, 25%, 50%, and 75%) as a prebiotic antioxidant on the probiotic viability, nutritional composition, and physicochemical and sensory properties of bio-flavored FCM with date syrup during storage at 4°C up to 21 days. The results obtained showed that replacing CM with ChM caused a decrease in the total solids (TS), fat, and ash contents and an increase in the fiber content of the bio-flavored FCM. The replacement of CM with ChM caused a significant (p ≤ 0.05) decrease in the values of potential of hydrogen (pH), viscosity, and acetaldehyde in the bio-flavored FCM; this decrease was proportional to the increase in the replacement rate. The total phenolic content (TPC), total flavonoids content (TFC), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition percentage of bio-flavored FCM significantly (p ≤ 0.05) increased with the replacement of CM with ChM. Also, ChM stimulated the growth of probiotic bacteria. The amino acid (AA) content of bio-flavored FCM was enhanced by replacing CM with ChM, where nonessential AA concentrations rose from 5.158 mg/g in control bio-flavored FCM to 54.964 mg/g in bio-flavored FCM + 50% ChM samples, a 50% increase over control bio-flavored FCM. The amount of essential AAs was also higher in the bio-flavored FCM + 50% ChM samples than in the control bio-flavored FCM, rising from 6.198 mg/g in the control bio-flavored FCM to 23.009 mg/g. The sensory evaluation revealed that samples with 50% ChM were preferred by panelists in sensory properties. The results obtained concluded that ChM could replace CM by up to 50% to enhance the quality of bio-flavored FCM and reduce production costs.
References
Agil R, Gaget A, Gliwa J, Avis TJ, Willmore WG, Hosseinian F. Lentils enhance probiotic growth in yogurt and provide added benefit of antioxidant protection. LWT-Food Sci Technol. 2013;50:45–9. https://doi.org/10.1016/j.lwt.2012.07.032
Aguilar‐Raymundo VG, Vélez‐Ruiz JF. Yoghurt‐type beverage with partial substitution of milk by a chickpea extract: Effect on physicochemical and flow properties. Int J Dairy Technol. 2019;72:266–74. https://doi.org/10.1111/1471-0307.12581
Aharon S, Hana B, Liel G, Ran H, Yoram K, Ilan S, et al. Total phenolic content and antioxidant activity of chickpea (Cicer arietinum L.) as affected by soaking and cooking conditions. Food Nutr Sci. 2011.
Al-Farsi K, Al-Habsi N, Al-Khusaibi M. The potential antioxidant properties of date products: A concise update. Can J Clin Nutr. 2018;6:84–104. https://doi.org/10.14206/canad.j.clin.nutr.2018.02.08
Allaith AAA. 2008. Antioxidant activity of Bahraini date palm (Phoenix dactylifera L.) fruit of various cultivars. Int J Food Sci Technol. 2008;43:1033–40. https://doi.org/10.1111/j.1365-2621.2007.01558.x
Aludatt MH, Ereifej K, Alothman AM, Almajwal A, Alkhalidy H, Al-Tawaha AR, et al. Variations of physical and chemical properties and mineral and vitamin composition of camel milk from eight locations in Jordan. J Food Agric Environ. 2010;8:16–20.
Aryana KJ. 2003. Folic acid fortified fat‐free plain set yoghurt. Int J Dairy Technol. 56:219–22. https://doi.org/10.1046/j.1471-0307.2003.00105.x
Atwaa E, Hassan M, Ramadan MF. Production of probiotic stirred yoghurt from camel milk and oat milk. J of Food and Dairy Sci Mansoura Univ. 2020;11:259–64. https://doi.org/10.21608/jfds.2020.118366
Atwaa ESH, Shahein MR, Alrashdi BM, Hassan MA, Alblihed MA, Dahran N, et al. Effects of fermented camel milk supplemented with Sidr fruit (Ziziphus spina-christi L.) pulp on hyperglycemia in streptozotocin-induced diabetic rats. Fermentation. 2022a;8:269. https://doi.org/10.3390/fermentation8060269
Atwaa ESH, Shahein MR, El-Sattar ESA, Hijazy HHA, Albrakati A, Elmahallawy EK. Bioactivity, physicochemical and sensory properties of probiotic yoghurt made from whole milk powder reconstituted in aqueous fennel extract. Fermentation. 2022b;8:52. https://doi.org/10.3390/fermentation8020052
Atwaa ESH, Shahein MR, Radwan HA, Mohammed NS, Aloraini MA, Albezrah NKA, et al. Antimicrobial activity of some plant extracts and their applications in homemade tomato paste and pasteurized cow milk as natural preservatives. Fermentation. 2022c;8:428. https://doi.org/10.3390/fermentation8090428
Atwaa ESH, Shahein MR, Raya-Álvarez E, El-Sattar A, Sayed E, Hassan MA, et al. Assessment of the physicochemical and sensory characteristics of fermented camel milk fortified with Cordia myxa and its biological effects against oxidative stress and hyperlipidemia in rats. Front nutr. 2023;10:1130224. https://doi.org/10.3389/fnut.2023.1130224
Bhinder S, Kaur A, Singh B, Yadav MP, Singh N. Proximate composition, amino acid profile, pasting and process characteristics of flour from different Tartary buckwheat varieties. Food Res Int. 2020;130:108946. https://doi.org/10.1016/j.foodres.2019.108946
Bhinder S, Singh B, Kaur A, Singh N, Kaur M, Kumari S, et al. Effect of infrared roasting on antioxidant activity, phenolic composition and Maillard reaction products of Tartary buckwheat varieties. Food Chem. 2019;285:240–51. https://doi.org/10.1016/j.foodchem.2019.01.141
Boukid F. Chickpea (Cicer arietinum L.) protein as a prospective plant‐based ingredient: A review. Int J Food Sci Technol. 2021;56:5435–44. https://doi.org/10.1111/ijfs.15046
Chandan RC, Gandhi A, Shah NP. Yogurt: Historical background, health benefits, and global trade: Yogurt in health and disease prevention. Elsevier. 2017;3–29. https://doi.org/10.1016/B978-0-12-805134-4.00001-8
Charalampopoulos D, Wang R, Pandiella S, Webb C. Application of cereals and cereal components in functional foods: A review. Int J Food Microbiol. 2002;79:131–41. https://doi.org/10.1016/S0168-1605(02)00187-3
Cruz A, Faria J, Walter E, Andrade R, Cavalcanti R, Oliveira CAFd, et al. Processing optimization of probiotic yogurt containing glucose oxidase using response surface methodology. J Dairy Sci. 2010;93:5059–68. https://doi.org/10.3168/jds.2010-3336
Drake M. Invited review: Sensory analysis of dairy foods. J Dairy Sci. 2007;90:4925–37. https://doi.org/10.3168/jds.2007-0332
Duarte CM, Mota J, Assunção R, Martins C, Ribeiro AC, Lima A, et al. New alternatives to milk from pulses: Chickpea and lupin beverages with improved digestibility and potential bioactivities for human health. Front nutr. 2022;9:852907. https://doi.org/10.3389/fnut.2022.852907
Dubeau S, Samson G, Tajmir-Riahi H-A. Dual effect of milk on the antioxidant capacity of green, Darjeeling, and English breakfast teas. Food Chem. 2010;122:539–45. https://doi.org/10.1016/j.foodchem.2010.03.005
Dufresne CJ, Farnworth ER. A review of latest research findings on the health promotion properties of tea. J nutr biochem. 2001;12:404–21. https://doi.org/10.1016/S0955-2863(01)00155-3
El-Karmany A, Abol-Ela M, Hassanein A. Production of a new yoghurt-like products fortified with some legumes and sweet, cereal crops and its evaluation of fungal load. J Food Dairy Sci, Mansoura Univ. 2013;4:133–48. https://doi.org/10.21608/jfds.2013.71788
Elbahnasi MA, Gerguis A, Abd El Galeel A, El-Zoghaby A. Quality assessment of yogurt enriched with oat and chickpea powders as source of dietary fibers. Zagazig J Agric Res. 2021;48:1043–54. https://doi.org/10.21608/zjar.2021.204544
Farahnaky A, Mardani M, Mesbahi G, Majzoobi M, Golmakani M. Some physicochemical properties of date syrup, conc entrate, and liquid sugar in comparison with sucrose solutions. J Agr Sci Tech. 2018;18:657–68
Fernandez-Orozco R, Frias J, Zielinski H, Muñoz R, Piskula MK, Kozlowska H, et al. Evaluation of bioprocesses to improve the antioxidant properties of chickpeas. LWT-Food Sci Technol. 2009;42:885–92. https://doi.org/10.1016/j.lwt.2008.10.013
Gad A, Kholif A, Sayed A. Evaluation of the nutritional value of functional yogurt resulting from combination of date palm syrup and skim milk. Am J Food Technol. 2010;5:250–9. https://doi.org/10.3923/ajft.2010.250.259
Ganbi H. Production of nutritious high quality date (Phoenix dactylifera) fruits syrup (Dibs) by using some novel technological approaches. J Appl Sci Res. 2012;1524–38.
Han IH, Baik BK. Oligosaccharide content and composition of legumes and their reduction by soaking, cooking, ultrasound, and high hydrostatic pressure. Cereal Chem. 2006;83:428–33. https://doi.org/10.1094/CC-83-0428
Hasani S, Sari AA, Heshmati A, Karami M. Physicochemical and sensory attributes assessment of functional low‐fat yogurt produced by incorporation of barley bran and Lactobacillus acidophilus. Food sci nutr. 2017;5:875–80. https://doi.org/10.1002/fsn3.470
Heimler D, Cimato A. Flavonoids from olive leaves (“Olea Europaea” L.) as affected by light. J Commod Sci. 2002;41:31–9.
Hernández-Hernández O, Muthaiyan A, Moreno FJ, Montilla A, Sanz ML, Ricke S. Effect of prebiotic carbohydrates on the growth and tolerance of Lactobacillus. Food Microbiol. 2012;30:355–61. https://doi.org/10.1016/j.fm.2011.12.022
Hill C, Guarner F, Reid G, Gibson GR, Merenstein DJ, Pot B, et al. Expert consensus document: The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol. 2014;11:506–14. https://doi.org/10.1038/nrgastro.2014.66
Horwitz W, editor. Official methods of analysis of AOAC International. Volume I, agricultural chemicals, contaminants, drugs. Gaithersburg (Maryland): AOAC International, 2010.
Hur SJ, Lee SY, Kim Y-C, Choi I, Kim G-B. Effect of fermentation on the antioxidant activity in plant-based foods. Food Chem. 2014;160:346–56. https://doi.org/10.1016/j.foodchem.2014.03.112
Hussein H, Awad S, El-Sayed I, Ibrahim A. Impact of chickpea as prebiotic, antioxidant and thickener agent of stirred bio-yoghurt. Ann Agric Sci. 2020;65:49–58. https://doi.org/10.1016/j.aoas.2020.03.001
Ishurd O, Kennedy JF. The anti-cancer activity of polysaccharide prepared from Libyan dates (Phoenix dactylifera L.). Carbohydr Polym. 2005;59:531–5. https://doi.org/10.1016/j.carbpol.2004.11.004
Ismail MM, Hamad MF, Elraghy EM. Using goat’s milk, barley flour, honey, and probiotic to manufacture of functional dairy product. Probiotics Antimicrob Proteins. 2018;10:677–91. https://doi.org/10.1007/s12602-017-9316-4
Jafarpour D, Amirzade A. Optimization of flavored drinking yoghurt formula containing date syrup and Lactobacillus plantarum and evaluation of some physicochemical characteristics and its overall acceptability. J Babol Univ Med Sci. 2018;20:.
Jafarpour D, Amirzadeh A, Maleki M, Mahmoudi M. Comparison of physicochemical properties and general acceptance of flavored drinking yogurt containing date and fig syrups. Foods Raw Mater. 2017;5:36–43. https://doi.org/10.21603/2308-4057-2017-2-36-43
Kaskous S. Importance of camel milk for human health. Emir J Food Agric. 2016;28(3):158–63. https://doi.org/10.9755/ejfa.2015-05-296
Kaur N, Singh B, Kaur A, Yadav MP. Impact of growing conditions on proximate, mineral, phenolic composition, amino acid profile, and antioxidant properties of black gram, mung bean, and chickpea microgreens. J Food Process Preserv. 2022;46:e16655. https://doi.org/10.1111/jfpp.16655
Kaur N, Singh B, Kaur A, Yadav MP, Singh N, Ahlawat AK, et al. Effect of growing conditions on proximate, mineral, amino acid, phenolic composition and antioxidant properties of wheatgrass from different wheat (Triticum aestivum L.) varieties. Food Chem. 2021;341:128201. https://doi.org/10.1016/j.foodchem.2020.128201
Korbekandi H, Mortazavian A, Iravani S. Technology and stability of probiotic in fermented milks. Probiotic and prebiotic foods: Technology., stability and benefits to the human health. Hauppauge, NY, USA: Nova Sciences Publishers; 2011. 131–167 pp.
Kumar H, Salminen S, Verhagen H, Rowland I, Heimbach J, Banares S, et al. Novel probiotics and prebiotics: Road to the market. Curr Opin Biotechnol. 2015;32:99–103. https://doi.org/10.1016/j.copbio.2014.11.021
Lees G, Jago G. Methods for the estimation of acetaldehyde in cultured dairy products. Aust J Dairy Technol. 1969;24:81–183.
Lim YY, Quah EP. Antioxidant properties of different cultivars of Portulaca oleracea. Food Chem. 2007;103:734–40. https://doi.org/10.1016/j.foodchem.2006.09.025
Lopez MJ, Mohiuddin SS. Biochemistry, essential amino acids. 2021; StatPearls Publishing, Treasure Island.
Lu Z, Lee P-R, Yang H. Chickpea flour and soy protein isolate interacted with κ-carrageenan via electrostatic interactions to form egg omelets analogue. Food Hydrocoll. 2022;130:107691. https://doi.org/10.1016/j.foodhyd.2022.107691
Mäkinen OE, Wanhalinna V, Zannini E, Arendt EK. Foods for special dietary needs: Non-dairy plant-based milk substitutes and fermented dairy-type products. Crit Rev Food Sci Nutr. 2016;56:339–49. https://doi.org/10.1080/10408398.2012.761950
Mang DY, Abdou AB, Njintang NY, Djiogue EM, Loura BB, Mbofung MC. Application of desirability-function and RSM to optimize antioxidant properties of mucuna milk. J Food Meas Charact. 2015;9:495–507. https://doi.org/10.1007/s11694-015-9258-zMaqsood S, Adiamo O, Ahmad M, Mudgil P. Bioactive compounds from date fruit and seed as potential nutraceutical and functional food ingredients. Food Chem. 2020;308:125522. https://doi.org/10.1016/j.foodchem.2019.125522
Martinez-Villaluenga C, Frias J, Gómez R, Vidal-Valverde C. Influence of addition of raffinose family oligosaccharides on probiotic survival in fermented milk during refrigerated storage. Int Dairy J. 2006;16:768–74. https://doi.org/10.1016/j.idairyj.2005.08.002
McClements DJ. Development of next-generation nutritionally fortified plant-based milk substitutes: Structural design principles. Foods. 2020;9:421. https://doi.org/10.3390/foods9040421
Meena S, Rajput Y, Sharma R. Comparative fat digestibility of goat, camel, cow and buffalo milk. Int Dairy J. 2014;35:153–6. https://doi.org/10.1016/j.idairyj.2013.11.009
Megías C, Cortés-Giraldo I, Alaiz M, Vioque J, Girón-Calle J. Isoflavones in chickpea (Cicer arietinum) protein concentrates. J Funct Foods. 2016;21:186–92. https://doi.org/10.1016/j.jff.2015.12.012
Mehta BM. Chemical composition of milk and milk products. Handbook Food Chem. 2015;511–53. https://doi.org/10.1007/978-3-642-36605-5_31
Miliauskas G, Venskutonis P, Van Beek T. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem. 2004;85:231–7. https://doi.org/10.1016/j.foodchem.2003.05.007
Mudgil P, Kamal H, Yuen GC, Maqsood S. Characterization and identification of novel antidiabetic and anti-obesity peptides from camel milk protein hydrolysates. Food Chem. 2018;259:46–54. https://doi.org/10.1016/j.foodchem.2018.03.082
Pittaway JK, Ahuja KD, Robertson IK, Ball MJ. Effects of a controlled diet supplemented with chickpeas on serum lipids, glucose tolerance, satiety and bowel function. J Am Coll Nutr. 2007;26:334–40. https://doi.org/10.1080/07315724.2007.10719620
Salem S., Meead G., and El-Rashody F. 2017. Physicochemical and sensory properties of ice cream made from camel milk and fortified with dates products. Int. J. Humanit. Arts Med. Sci. 5:29–40.
Salfinger Y., and Tortorello ML. 2015. Compendium of methods for the microbiological examination of foods: American Public Health Association. https://doi.org/10.2105/MBEF.0222
Shah S., Ullah F., and Munir I. 2020. Biochemical characterization for determination of genetic distances among different indigenous chickpea (Cicer arietinum L.) varieties of North-West Pakistan. Braz. J. Biol. 81:977–988. https://doi.org/10.1590/1519-6984.232747
Shahein MR., Atwaa E-SH., Babalghith AO., Alrashdi BM., Radwan HA., Umair M., Abdalmegeed D., Mahfouz H., Dahran N., and Cacciotti I. 2022a. Impact of incorporating the aqueous extract of hawthorn (C. oxyanatha) leaves on yogurt properties and its therapeutic effects against oxidative stress induced by carbon tetrachloride in rats. Fermentation 8:200. https://doi.org/10.3390/fermentation8050200
Shahein MR., Atwaa E-SH., Babalghith AO., Alrashdi BM., Radwan HA., Umair M., Abdalmegeed D., Mahfouz H., Dahran N., Cacciotti I., and Elmahallawy EK. 2022b. Impact of Incorporating the Aqueous Extract of Hawthorn (C. oxyanatha) leaves on Yogurt Properties and Its Therapeutic Effects against Oxidative Stress Induced by Carbon Tetrachloride in Rats. Fermentation 8:200. https://doi.org/10.3390/fermentation8050200
Shahein MR., Atwaa ESH., Alrashdi BM., Ramadan MF., Abd El-Sattar ES., Siam AAH., Alblihed MA., and Elmahallawy EK. 2022c. Effect of fermented camel milk containing pumpkin seed milk on the oxidative stress induced by carbon tetrachloride in experimental rats. Fermentation 8:223. https://doi.org/10.3390/fermentation8050223
Shahein MR., Atwaa ESH., El-Zahar KM., Elmaadawy AA., Hijazy HHA., Sitohy MZ., Albrakati A., and Elmahallawy EK. 2022d. Remedial Action of Yoghurt Enriched with Watermelon Seed Milk on Renal Injured Hyperuricemic Rats. Fermentation 8:41. https://doi.org/10.3390/fermentation8020041
Shahein MR., Atwaa ESH., Elkot WF., Hijazy HHA., Kassab RB., Alblihed MA., and Elmahallawy EK. 2022e. The impact of date syrup on the physicochemical., microbiological., and sensory properties., and antioxidant activity of bio-fermented camel milk. Fermentation 8:192. https://doi.org/10.3390/fermentation8050192
Shahein MR., Atwaa ESH., Radwan HA., Elmeligy AA., Hafiz AA., Albrakati A., and Elmahallawy EK. 2022f. Production of a Yogurt Drink Enriched with Golden Berry (Physalispubescens L.) Juice and Its Therapeutic Effect on Hepatitis in Rats. Fermentation 8:112. https://doi.org/10.3390/fermentation8030112
Shahein MR., Elkot WF., Albezrah NKA., Abdel-Hafez LJM., Alharbi MA., Massoud D., and Elmahallawy EK. 2022g. Insights into the Microbiological and Physicochemical Properties of Bio-Frozen Yoghurt Made with Probiotic Strains in Combination with Jerusalem Artichoke Tubers Powder. Fermentation 8:390. https://doi.org/10.3390/fermentation8080390
Shahein MR., Raya-Álvarez E., Hassan MA., Hashim MA., Dahran N., El-Khadragy MF., Agil A., and Elmahallawy EK. 2023. Assessment of the physicochemical and sensory characteristics of fermented camel milk fortified with Cordia myxa and its biological effects against oxidative stress and hyperlipidemia in rats. Front. nutr. 10:1130224. https://doi.org/10.3389/fnut.2023.1130224
Sim SY., Hua XY., and Henry CJ. 2020. A novel approach to structure plant-based yogurts using high pressure processing. Foods 9:1126. https://doi.org/10.3390/foods9081126
Singleton VL., Orthofer R., and Lamuela-Raventós RM. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in enzymology: Elsevier. 152–178. https://doi.org/10.1016/S0076-6879(99)99017-1
Solanki D., and Hati S. 2018. Fermented camel milk: A Review on its bio-functional properties. Emir. J. Food Agric. 268–274. https://doi.org/10.9755/ejfa.2018.v30.i4.1661
Swelam S., Zommara MA., Abd El-Aziz AE-AM., Elgammal NA., Baty RS., and Elmahallawy EK. 2021. Insights into Chufa Milk Frozen Yoghurt as Cheap Functional Frozen Yoghurt with High Nutritional Value. Fermentation 7:255. https://doi.org/10.3390/fermentation7040255
Tamime AY., and Robinson RK. 2007. Tamime and Robinson's yoghurt: science and technology: Elsevier. https://doi.org/10.1201/NOE1420044539
Tammam A., Mansour A., Salman K., and El-Gazzar F. 2013. Preparation., and properties of bio-yoghurt containing date syrup (dibis). Egypt J Dairy Sci. 41:69–76.
Terpinc P., Čeh B., Ulrih NP., and Abramovič H. 2012. Studies of the correlation between antioxidant properties and the total phenolic content of different oil cake extracts. Ind. Crops Prod. 39:210–217. https://doi.org/10.1016/j.indcrop.2012.02.023
Trigueros L., Wojdyło A., and Sendra E. 2014. Antioxidant activity and protein–polyphenol interactions in a pomegranate (Punica granatum L.) yogurt. J. Agric. Food Chem. 62:6417–6425. https://doi.org/10.1021/jf501503h
Vallath A., Shanmugam A., and Rawson A. 2021. Evaluation of physicochemical and organoleptic properties of plant based beverage developed from chickpea. Pharm. Innov. 10:1871–1875.
Wang S., Chelikani V., and Serventi L. 2018. Evaluation of chickpea as alternative to soy in plant-based beverages., fresh and fermented. LWT. 97:570–572. https://doi.org/10.1016/j.lwt.2018.07.067
Xu M., Jin Z., Gu Z., Rao J., and Chen B. 2020. Changes in odor characteristics of pulse protein isolates from germinated chickpea., lentil., and yellow pea: Role of lipoxygenase and free radicals. Food Chem. 314:126184. https://doi.org/10.1016/j.foodchem.2020.126184
Yogurt F. CFR 131.200., code of federal regulations. US Department of Health and Human Services.
Yu M., Gouvinhas I., Rocha J., and Barros AI. 2021. Phytochemical and antioxidant analysis of medicinal and food plants towards bioactive food and pharmaceutical resources. Sci. Rep. 11:10041. https://doi.org/10.1038/s41598-021-89437-4
Zhang P., Tang F., Cai W., Zhao X., and Shan C. 2022a. Evaluating the effect of lactic acid bacteria fermentation on quality., aroma., and metabolites of chickpea milk. Front. nutr. 9:1069714. https://doi.org/10.3389/fnut.2022.1069714
Zhang X., Zhang S., Xie B., and Sun Z. 2022b. Regulation on structure rheological properties and aroma volatile compounds of fermented chickpea milk by enzymatic catalysis. International Journal of Food Science & Technology 57:3665–3680. https://doi.org/10.1111/ijfs.15692
Zhu M-X., Xu C., and Hao YP. 2023. Lactobacillus fermentation improved the nutrient value and hypoglycemic activity of chickpea milk. Emir. J. Food Agric. 35(3): 242–250.
Zommara MA., Bedeer EG., Elmahallawy EK., Hafiz AA., Albrakati A., and Swelam S. 2022. Preliminary Studies of Bio-Fortification of Yoghurt with Chromium. Fermentation 8:727. https://doi.org/10.3390/fermentation8120727
Aguilar‐Raymundo VG, Vélez‐Ruiz JF. Yoghurt‐type beverage with partial substitution of milk by a chickpea extract: Effect on physicochemical and flow properties. Int J Dairy Technol. 2019;72:266–74. https://doi.org/10.1111/1471-0307.12581
Aharon S, Hana B, Liel G, Ran H, Yoram K, Ilan S, et al. Total phenolic content and antioxidant activity of chickpea (Cicer arietinum L.) as affected by soaking and cooking conditions. Food Nutr Sci. 2011.
Al-Farsi K, Al-Habsi N, Al-Khusaibi M. The potential antioxidant properties of date products: A concise update. Can J Clin Nutr. 2018;6:84–104. https://doi.org/10.14206/canad.j.clin.nutr.2018.02.08
Allaith AAA. 2008. Antioxidant activity of Bahraini date palm (Phoenix dactylifera L.) fruit of various cultivars. Int J Food Sci Technol. 2008;43:1033–40. https://doi.org/10.1111/j.1365-2621.2007.01558.x
Aludatt MH, Ereifej K, Alothman AM, Almajwal A, Alkhalidy H, Al-Tawaha AR, et al. Variations of physical and chemical properties and mineral and vitamin composition of camel milk from eight locations in Jordan. J Food Agric Environ. 2010;8:16–20.
Aryana KJ. 2003. Folic acid fortified fat‐free plain set yoghurt. Int J Dairy Technol. 56:219–22. https://doi.org/10.1046/j.1471-0307.2003.00105.x
Atwaa E, Hassan M, Ramadan MF. Production of probiotic stirred yoghurt from camel milk and oat milk. J of Food and Dairy Sci Mansoura Univ. 2020;11:259–64. https://doi.org/10.21608/jfds.2020.118366
Atwaa ESH, Shahein MR, Alrashdi BM, Hassan MA, Alblihed MA, Dahran N, et al. Effects of fermented camel milk supplemented with Sidr fruit (Ziziphus spina-christi L.) pulp on hyperglycemia in streptozotocin-induced diabetic rats. Fermentation. 2022a;8:269. https://doi.org/10.3390/fermentation8060269
Atwaa ESH, Shahein MR, El-Sattar ESA, Hijazy HHA, Albrakati A, Elmahallawy EK. Bioactivity, physicochemical and sensory properties of probiotic yoghurt made from whole milk powder reconstituted in aqueous fennel extract. Fermentation. 2022b;8:52. https://doi.org/10.3390/fermentation8020052
Atwaa ESH, Shahein MR, Radwan HA, Mohammed NS, Aloraini MA, Albezrah NKA, et al. Antimicrobial activity of some plant extracts and their applications in homemade tomato paste and pasteurized cow milk as natural preservatives. Fermentation. 2022c;8:428. https://doi.org/10.3390/fermentation8090428
Atwaa ESH, Shahein MR, Raya-Álvarez E, El-Sattar A, Sayed E, Hassan MA, et al. Assessment of the physicochemical and sensory characteristics of fermented camel milk fortified with Cordia myxa and its biological effects against oxidative stress and hyperlipidemia in rats. Front nutr. 2023;10:1130224. https://doi.org/10.3389/fnut.2023.1130224
Bhinder S, Kaur A, Singh B, Yadav MP, Singh N. Proximate composition, amino acid profile, pasting and process characteristics of flour from different Tartary buckwheat varieties. Food Res Int. 2020;130:108946. https://doi.org/10.1016/j.foodres.2019.108946
Bhinder S, Singh B, Kaur A, Singh N, Kaur M, Kumari S, et al. Effect of infrared roasting on antioxidant activity, phenolic composition and Maillard reaction products of Tartary buckwheat varieties. Food Chem. 2019;285:240–51. https://doi.org/10.1016/j.foodchem.2019.01.141
Boukid F. Chickpea (Cicer arietinum L.) protein as a prospective plant‐based ingredient: A review. Int J Food Sci Technol. 2021;56:5435–44. https://doi.org/10.1111/ijfs.15046
Chandan RC, Gandhi A, Shah NP. Yogurt: Historical background, health benefits, and global trade: Yogurt in health and disease prevention. Elsevier. 2017;3–29. https://doi.org/10.1016/B978-0-12-805134-4.00001-8
Charalampopoulos D, Wang R, Pandiella S, Webb C. Application of cereals and cereal components in functional foods: A review. Int J Food Microbiol. 2002;79:131–41. https://doi.org/10.1016/S0168-1605(02)00187-3
Cruz A, Faria J, Walter E, Andrade R, Cavalcanti R, Oliveira CAFd, et al. Processing optimization of probiotic yogurt containing glucose oxidase using response surface methodology. J Dairy Sci. 2010;93:5059–68. https://doi.org/10.3168/jds.2010-3336
Drake M. Invited review: Sensory analysis of dairy foods. J Dairy Sci. 2007;90:4925–37. https://doi.org/10.3168/jds.2007-0332
Duarte CM, Mota J, Assunção R, Martins C, Ribeiro AC, Lima A, et al. New alternatives to milk from pulses: Chickpea and lupin beverages with improved digestibility and potential bioactivities for human health. Front nutr. 2022;9:852907. https://doi.org/10.3389/fnut.2022.852907
Dubeau S, Samson G, Tajmir-Riahi H-A. Dual effect of milk on the antioxidant capacity of green, Darjeeling, and English breakfast teas. Food Chem. 2010;122:539–45. https://doi.org/10.1016/j.foodchem.2010.03.005
Dufresne CJ, Farnworth ER. A review of latest research findings on the health promotion properties of tea. J nutr biochem. 2001;12:404–21. https://doi.org/10.1016/S0955-2863(01)00155-3
El-Karmany A, Abol-Ela M, Hassanein A. Production of a new yoghurt-like products fortified with some legumes and sweet, cereal crops and its evaluation of fungal load. J Food Dairy Sci, Mansoura Univ. 2013;4:133–48. https://doi.org/10.21608/jfds.2013.71788
Elbahnasi MA, Gerguis A, Abd El Galeel A, El-Zoghaby A. Quality assessment of yogurt enriched with oat and chickpea powders as source of dietary fibers. Zagazig J Agric Res. 2021;48:1043–54. https://doi.org/10.21608/zjar.2021.204544
Farahnaky A, Mardani M, Mesbahi G, Majzoobi M, Golmakani M. Some physicochemical properties of date syrup, conc entrate, and liquid sugar in comparison with sucrose solutions. J Agr Sci Tech. 2018;18:657–68
Fernandez-Orozco R, Frias J, Zielinski H, Muñoz R, Piskula MK, Kozlowska H, et al. Evaluation of bioprocesses to improve the antioxidant properties of chickpeas. LWT-Food Sci Technol. 2009;42:885–92. https://doi.org/10.1016/j.lwt.2008.10.013
Gad A, Kholif A, Sayed A. Evaluation of the nutritional value of functional yogurt resulting from combination of date palm syrup and skim milk. Am J Food Technol. 2010;5:250–9. https://doi.org/10.3923/ajft.2010.250.259
Ganbi H. Production of nutritious high quality date (Phoenix dactylifera) fruits syrup (Dibs) by using some novel technological approaches. J Appl Sci Res. 2012;1524–38.
Han IH, Baik BK. Oligosaccharide content and composition of legumes and their reduction by soaking, cooking, ultrasound, and high hydrostatic pressure. Cereal Chem. 2006;83:428–33. https://doi.org/10.1094/CC-83-0428
Hasani S, Sari AA, Heshmati A, Karami M. Physicochemical and sensory attributes assessment of functional low‐fat yogurt produced by incorporation of barley bran and Lactobacillus acidophilus. Food sci nutr. 2017;5:875–80. https://doi.org/10.1002/fsn3.470
Heimler D, Cimato A. Flavonoids from olive leaves (“Olea Europaea” L.) as affected by light. J Commod Sci. 2002;41:31–9.
Hernández-Hernández O, Muthaiyan A, Moreno FJ, Montilla A, Sanz ML, Ricke S. Effect of prebiotic carbohydrates on the growth and tolerance of Lactobacillus. Food Microbiol. 2012;30:355–61. https://doi.org/10.1016/j.fm.2011.12.022
Hill C, Guarner F, Reid G, Gibson GR, Merenstein DJ, Pot B, et al. Expert consensus document: The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol. 2014;11:506–14. https://doi.org/10.1038/nrgastro.2014.66
Horwitz W, editor. Official methods of analysis of AOAC International. Volume I, agricultural chemicals, contaminants, drugs. Gaithersburg (Maryland): AOAC International, 2010.
Hur SJ, Lee SY, Kim Y-C, Choi I, Kim G-B. Effect of fermentation on the antioxidant activity in plant-based foods. Food Chem. 2014;160:346–56. https://doi.org/10.1016/j.foodchem.2014.03.112
Hussein H, Awad S, El-Sayed I, Ibrahim A. Impact of chickpea as prebiotic, antioxidant and thickener agent of stirred bio-yoghurt. Ann Agric Sci. 2020;65:49–58. https://doi.org/10.1016/j.aoas.2020.03.001
Ishurd O, Kennedy JF. The anti-cancer activity of polysaccharide prepared from Libyan dates (Phoenix dactylifera L.). Carbohydr Polym. 2005;59:531–5. https://doi.org/10.1016/j.carbpol.2004.11.004
Ismail MM, Hamad MF, Elraghy EM. Using goat’s milk, barley flour, honey, and probiotic to manufacture of functional dairy product. Probiotics Antimicrob Proteins. 2018;10:677–91. https://doi.org/10.1007/s12602-017-9316-4
Jafarpour D, Amirzade A. Optimization of flavored drinking yoghurt formula containing date syrup and Lactobacillus plantarum and evaluation of some physicochemical characteristics and its overall acceptability. J Babol Univ Med Sci. 2018;20:.
Jafarpour D, Amirzadeh A, Maleki M, Mahmoudi M. Comparison of physicochemical properties and general acceptance of flavored drinking yogurt containing date and fig syrups. Foods Raw Mater. 2017;5:36–43. https://doi.org/10.21603/2308-4057-2017-2-36-43
Kaskous S. Importance of camel milk for human health. Emir J Food Agric. 2016;28(3):158–63. https://doi.org/10.9755/ejfa.2015-05-296
Kaur N, Singh B, Kaur A, Yadav MP. Impact of growing conditions on proximate, mineral, phenolic composition, amino acid profile, and antioxidant properties of black gram, mung bean, and chickpea microgreens. J Food Process Preserv. 2022;46:e16655. https://doi.org/10.1111/jfpp.16655
Kaur N, Singh B, Kaur A, Yadav MP, Singh N, Ahlawat AK, et al. Effect of growing conditions on proximate, mineral, amino acid, phenolic composition and antioxidant properties of wheatgrass from different wheat (Triticum aestivum L.) varieties. Food Chem. 2021;341:128201. https://doi.org/10.1016/j.foodchem.2020.128201
Korbekandi H, Mortazavian A, Iravani S. Technology and stability of probiotic in fermented milks. Probiotic and prebiotic foods: Technology., stability and benefits to the human health. Hauppauge, NY, USA: Nova Sciences Publishers; 2011. 131–167 pp.
Kumar H, Salminen S, Verhagen H, Rowland I, Heimbach J, Banares S, et al. Novel probiotics and prebiotics: Road to the market. Curr Opin Biotechnol. 2015;32:99–103. https://doi.org/10.1016/j.copbio.2014.11.021
Lees G, Jago G. Methods for the estimation of acetaldehyde in cultured dairy products. Aust J Dairy Technol. 1969;24:81–183.
Lim YY, Quah EP. Antioxidant properties of different cultivars of Portulaca oleracea. Food Chem. 2007;103:734–40. https://doi.org/10.1016/j.foodchem.2006.09.025
Lopez MJ, Mohiuddin SS. Biochemistry, essential amino acids. 2021; StatPearls Publishing, Treasure Island.
Lu Z, Lee P-R, Yang H. Chickpea flour and soy protein isolate interacted with κ-carrageenan via electrostatic interactions to form egg omelets analogue. Food Hydrocoll. 2022;130:107691. https://doi.org/10.1016/j.foodhyd.2022.107691
Mäkinen OE, Wanhalinna V, Zannini E, Arendt EK. Foods for special dietary needs: Non-dairy plant-based milk substitutes and fermented dairy-type products. Crit Rev Food Sci Nutr. 2016;56:339–49. https://doi.org/10.1080/10408398.2012.761950
Mang DY, Abdou AB, Njintang NY, Djiogue EM, Loura BB, Mbofung MC. Application of desirability-function and RSM to optimize antioxidant properties of mucuna milk. J Food Meas Charact. 2015;9:495–507. https://doi.org/10.1007/s11694-015-9258-zMaqsood S, Adiamo O, Ahmad M, Mudgil P. Bioactive compounds from date fruit and seed as potential nutraceutical and functional food ingredients. Food Chem. 2020;308:125522. https://doi.org/10.1016/j.foodchem.2019.125522
Martinez-Villaluenga C, Frias J, Gómez R, Vidal-Valverde C. Influence of addition of raffinose family oligosaccharides on probiotic survival in fermented milk during refrigerated storage. Int Dairy J. 2006;16:768–74. https://doi.org/10.1016/j.idairyj.2005.08.002
McClements DJ. Development of next-generation nutritionally fortified plant-based milk substitutes: Structural design principles. Foods. 2020;9:421. https://doi.org/10.3390/foods9040421
Meena S, Rajput Y, Sharma R. Comparative fat digestibility of goat, camel, cow and buffalo milk. Int Dairy J. 2014;35:153–6. https://doi.org/10.1016/j.idairyj.2013.11.009
Megías C, Cortés-Giraldo I, Alaiz M, Vioque J, Girón-Calle J. Isoflavones in chickpea (Cicer arietinum) protein concentrates. J Funct Foods. 2016;21:186–92. https://doi.org/10.1016/j.jff.2015.12.012
Mehta BM. Chemical composition of milk and milk products. Handbook Food Chem. 2015;511–53. https://doi.org/10.1007/978-3-642-36605-5_31
Miliauskas G, Venskutonis P, Van Beek T. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem. 2004;85:231–7. https://doi.org/10.1016/j.foodchem.2003.05.007
Mudgil P, Kamal H, Yuen GC, Maqsood S. Characterization and identification of novel antidiabetic and anti-obesity peptides from camel milk protein hydrolysates. Food Chem. 2018;259:46–54. https://doi.org/10.1016/j.foodchem.2018.03.082
Pittaway JK, Ahuja KD, Robertson IK, Ball MJ. Effects of a controlled diet supplemented with chickpeas on serum lipids, glucose tolerance, satiety and bowel function. J Am Coll Nutr. 2007;26:334–40. https://doi.org/10.1080/07315724.2007.10719620
Salem S., Meead G., and El-Rashody F. 2017. Physicochemical and sensory properties of ice cream made from camel milk and fortified with dates products. Int. J. Humanit. Arts Med. Sci. 5:29–40.
Salfinger Y., and Tortorello ML. 2015. Compendium of methods for the microbiological examination of foods: American Public Health Association. https://doi.org/10.2105/MBEF.0222
Shah S., Ullah F., and Munir I. 2020. Biochemical characterization for determination of genetic distances among different indigenous chickpea (Cicer arietinum L.) varieties of North-West Pakistan. Braz. J. Biol. 81:977–988. https://doi.org/10.1590/1519-6984.232747
Shahein MR., Atwaa E-SH., Babalghith AO., Alrashdi BM., Radwan HA., Umair M., Abdalmegeed D., Mahfouz H., Dahran N., and Cacciotti I. 2022a. Impact of incorporating the aqueous extract of hawthorn (C. oxyanatha) leaves on yogurt properties and its therapeutic effects against oxidative stress induced by carbon tetrachloride in rats. Fermentation 8:200. https://doi.org/10.3390/fermentation8050200
Shahein MR., Atwaa E-SH., Babalghith AO., Alrashdi BM., Radwan HA., Umair M., Abdalmegeed D., Mahfouz H., Dahran N., Cacciotti I., and Elmahallawy EK. 2022b. Impact of Incorporating the Aqueous Extract of Hawthorn (C. oxyanatha) leaves on Yogurt Properties and Its Therapeutic Effects against Oxidative Stress Induced by Carbon Tetrachloride in Rats. Fermentation 8:200. https://doi.org/10.3390/fermentation8050200
Shahein MR., Atwaa ESH., Alrashdi BM., Ramadan MF., Abd El-Sattar ES., Siam AAH., Alblihed MA., and Elmahallawy EK. 2022c. Effect of fermented camel milk containing pumpkin seed milk on the oxidative stress induced by carbon tetrachloride in experimental rats. Fermentation 8:223. https://doi.org/10.3390/fermentation8050223
Shahein MR., Atwaa ESH., El-Zahar KM., Elmaadawy AA., Hijazy HHA., Sitohy MZ., Albrakati A., and Elmahallawy EK. 2022d. Remedial Action of Yoghurt Enriched with Watermelon Seed Milk on Renal Injured Hyperuricemic Rats. Fermentation 8:41. https://doi.org/10.3390/fermentation8020041
Shahein MR., Atwaa ESH., Elkot WF., Hijazy HHA., Kassab RB., Alblihed MA., and Elmahallawy EK. 2022e. The impact of date syrup on the physicochemical., microbiological., and sensory properties., and antioxidant activity of bio-fermented camel milk. Fermentation 8:192. https://doi.org/10.3390/fermentation8050192
Shahein MR., Atwaa ESH., Radwan HA., Elmeligy AA., Hafiz AA., Albrakati A., and Elmahallawy EK. 2022f. Production of a Yogurt Drink Enriched with Golden Berry (Physalispubescens L.) Juice and Its Therapeutic Effect on Hepatitis in Rats. Fermentation 8:112. https://doi.org/10.3390/fermentation8030112
Shahein MR., Elkot WF., Albezrah NKA., Abdel-Hafez LJM., Alharbi MA., Massoud D., and Elmahallawy EK. 2022g. Insights into the Microbiological and Physicochemical Properties of Bio-Frozen Yoghurt Made with Probiotic Strains in Combination with Jerusalem Artichoke Tubers Powder. Fermentation 8:390. https://doi.org/10.3390/fermentation8080390
Shahein MR., Raya-Álvarez E., Hassan MA., Hashim MA., Dahran N., El-Khadragy MF., Agil A., and Elmahallawy EK. 2023. Assessment of the physicochemical and sensory characteristics of fermented camel milk fortified with Cordia myxa and its biological effects against oxidative stress and hyperlipidemia in rats. Front. nutr. 10:1130224. https://doi.org/10.3389/fnut.2023.1130224
Sim SY., Hua XY., and Henry CJ. 2020. A novel approach to structure plant-based yogurts using high pressure processing. Foods 9:1126. https://doi.org/10.3390/foods9081126
Singleton VL., Orthofer R., and Lamuela-Raventós RM. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in enzymology: Elsevier. 152–178. https://doi.org/10.1016/S0076-6879(99)99017-1
Solanki D., and Hati S. 2018. Fermented camel milk: A Review on its bio-functional properties. Emir. J. Food Agric. 268–274. https://doi.org/10.9755/ejfa.2018.v30.i4.1661
Swelam S., Zommara MA., Abd El-Aziz AE-AM., Elgammal NA., Baty RS., and Elmahallawy EK. 2021. Insights into Chufa Milk Frozen Yoghurt as Cheap Functional Frozen Yoghurt with High Nutritional Value. Fermentation 7:255. https://doi.org/10.3390/fermentation7040255
Tamime AY., and Robinson RK. 2007. Tamime and Robinson's yoghurt: science and technology: Elsevier. https://doi.org/10.1201/NOE1420044539
Tammam A., Mansour A., Salman K., and El-Gazzar F. 2013. Preparation., and properties of bio-yoghurt containing date syrup (dibis). Egypt J Dairy Sci. 41:69–76.
Terpinc P., Čeh B., Ulrih NP., and Abramovič H. 2012. Studies of the correlation between antioxidant properties and the total phenolic content of different oil cake extracts. Ind. Crops Prod. 39:210–217. https://doi.org/10.1016/j.indcrop.2012.02.023
Trigueros L., Wojdyło A., and Sendra E. 2014. Antioxidant activity and protein–polyphenol interactions in a pomegranate (Punica granatum L.) yogurt. J. Agric. Food Chem. 62:6417–6425. https://doi.org/10.1021/jf501503h
Vallath A., Shanmugam A., and Rawson A. 2021. Evaluation of physicochemical and organoleptic properties of plant based beverage developed from chickpea. Pharm. Innov. 10:1871–1875.
Wang S., Chelikani V., and Serventi L. 2018. Evaluation of chickpea as alternative to soy in plant-based beverages., fresh and fermented. LWT. 97:570–572. https://doi.org/10.1016/j.lwt.2018.07.067
Xu M., Jin Z., Gu Z., Rao J., and Chen B. 2020. Changes in odor characteristics of pulse protein isolates from germinated chickpea., lentil., and yellow pea: Role of lipoxygenase and free radicals. Food Chem. 314:126184. https://doi.org/10.1016/j.foodchem.2020.126184
Yogurt F. CFR 131.200., code of federal regulations. US Department of Health and Human Services.
Yu M., Gouvinhas I., Rocha J., and Barros AI. 2021. Phytochemical and antioxidant analysis of medicinal and food plants towards bioactive food and pharmaceutical resources. Sci. Rep. 11:10041. https://doi.org/10.1038/s41598-021-89437-4
Zhang P., Tang F., Cai W., Zhao X., and Shan C. 2022a. Evaluating the effect of lactic acid bacteria fermentation on quality., aroma., and metabolites of chickpea milk. Front. nutr. 9:1069714. https://doi.org/10.3389/fnut.2022.1069714
Zhang X., Zhang S., Xie B., and Sun Z. 2022b. Regulation on structure rheological properties and aroma volatile compounds of fermented chickpea milk by enzymatic catalysis. International Journal of Food Science & Technology 57:3665–3680. https://doi.org/10.1111/ijfs.15692
Zhu M-X., Xu C., and Hao YP. 2023. Lactobacillus fermentation improved the nutrient value and hypoglycemic activity of chickpea milk. Emir. J. Food Agric. 35(3): 242–250.
Zommara MA., Bedeer EG., Elmahallawy EK., Hafiz AA., Albrakati A., and Swelam S. 2022. Preliminary Studies of Bio-Fortification of Yoghurt with Chromium. Fermentation 8:727. https://doi.org/10.3390/fermentation8120727
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Abdel-Rauf , H. A. H. ., Raya-Álvarez, E. ., Al-Nashwi, A. A. ., Alkhudhayri, D. A. ., Khalifa, S. A. ., Shokry, E. S. ., Aljehani, A. A. ., Albrakati, A. ., Agil, A. ., & Elmahallawy, E. K. (2025). Nutritional composition, physicochemical, microbiological, and sensory properties of flavored biofermented camel milk containing chickpea milk. Italian Journal of Food Science, 37(3), 174-189. https://doi.org/10.15586/ijfs.v37i3.2863
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Abdel-Rauf , H. A. H. ., Raya-Álvarez, E. ., Al-Nashwi, A. A. ., Alkhudhayri, D. A. ., Khalifa, S. A. ., Shokry, E. S. ., Aljehani, A. A. ., Albrakati, A. ., Agil, A. ., & Elmahallawy, E. K. (2025). Nutritional composition, physicochemical, microbiological, and sensory properties of flavored biofermented camel milk containing chickpea milk. Italian Journal of Food Science, 37(3), 174-189. https://doi.org/10.15586/ijfs.v37i3.2863