Paulownia tomentosa flower polysaccharide as an effective immunopotentiator to enhance immune responses for Newcastle disease vaccine in mice

Main Article Content

Xiaolan Chen
Junjie Jin
Fuxing Hao
Haifeng Yang
Hongxiang Sun
Chunmao Jiang


immune responses, immunopotentiator, Newcastle disease vaccine, Paulownia tomentosa flower polysaccharides


To investigate the immunomodulatory activity and explore the mechanism of Paulownia tomentosa flower polysaccharides (PTFP). PTFP was orally administrated to mice for seven successive days before and after Newcastle disease vaccination. The results demonstrated that compared with the vaccine control (VC) group, PTFP enhanced the inhibition of hemagglutination assay antibody titers, promoted the antigen-specific immunoglobulin (Ig)G, IgG1, IgG2a, and IgG2b antibodies responses, enhanced proliferation of spleen T and B lymphocytes, increased the secretions of interferon-γ and interleukin-10 cytokines of spleen lymphocytes, and promoted the activation of natural killer cells. Therefore, PTFP, as an effective immunopotentiator, could induce a mixed T-helper (Th)1 and Th2 immune responses and an innate immune response.

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Chen X., Han W., Wang G., Zhao X. 2020. Application prospect of polysaccharides in the development of anti-novel coronavirus drugs and vaccines. Int. J. Biol. Macromol. 164:331–343. 10.1016/j.ijbiomac.2020.07.106

Chen X., Yang H., Jia J., Chen Y., Wang J., Chen H., et al. 2021. Mulberry leaf polysaccharide supplementation contributes to enhancing the respiratory mucosal barrier immune response in Newcastle disease virus-vaccinated chicks. Poult Sci. 2021;100:592–602. 10.1016/j.psj.2020.11.039

Courant, T., Bayon, E., Reynaud-Dougier, H. L., Villiers, C., Menneteau M., Marche P.N., et al. 2017. Tailoring nanostructured lipid carriers for the delivery of protein antigens: physicochemical properties versus immunogenicity studies. Biomaterials. 136:29–42. 10.1016/j.biomaterials.2017.05.001

Dai B., Hu Z., Li H., Yan C., Zhang L. 2015. Simultaneous determination of six flavonoids from Paulownia tomentosa flower extract in rat plasma by LC-MS/MS and its application to a pharmacokinetic study. J. Chromatogr. B. 978-979:54–61. 10.1016/j.jchromb.2014.11.021

Feng H., Du X., Tang J., Cao X., Han X., Chen Z., et al. 2013. Enhancement of the immune responses to foot-and-mouth disease vaccination in mice by oral administration of a novel polysaccharide from the roots of Radix Cyathulae officinalis Kuan (RC). Cell. Immunol. 281(2):111–121. 10.1016/j.cellimm.2013.02.004

Gan T., Feng C., Lan H., Yang R., Zhang J., Li C., et al. 2021. Comparison of the structure and immunomodulatory activity of polysaccharides from fresh and dried longan. J. Funct. Foods. 2021;76:104323. 10.1016/j.jff.2020.104323

Huang R., Shen M., Yu Y., Liu X., Xie J. 2020. Physicochemical characterization and immunomodulatory activity of sulfated Chinese yam polysaccharide. Int. J. Biol. Macromol. 165:635–644. 10.1016/j.ijbiomac.2020.09.213

Huang Y., Jiang C., Hu Y., Zhao X., Shi C., Yu Y., et al. 2013. Immunoenhancement effect of Rehmannia glutinosa polysaccharide on lymphocyte proliferation and dendritic cell. Carbohydr. Polym. 2013;96(2):516–521. 10.1016/j.carbpol.2013.04.018

Kumar S., Kesharwani S.S., Kuppast B., Bakkari M.A., Tummala H. 2017. Pathogen-mimicking vaccine delivery system designed with a bioactive polymer (inulin acetate) for robust humoral and cellular immune responses. J Control Release. 261:263–274. 10.1016/j.jconrel.2017.06.026

Lee J.W., Seo K.H., Ryu H.W., Yuk H.J., Park H.A., Lim Y., et al. 2018. Anti-inflammatory effect of stem bark of Paulownia tomentosa Steud. in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and LPS-induced murine model of acute lung injury. J. Ethnopharmacol. 210:23–30. 10.1016/j.jep.2017.08.028

Liu C., Ma J., Sun J., Cheng C., Feng Z., Jiang H., et al. 2017. Flavonoid-rich extract of paulownia fortunei flowers attenuates diet-induced hyperlipidemia, hepatic steatosis and insulin resistance in obesity mice by AMPK pathway. nutrients. 9(9):959. 10.3390/nu9090959

Liu Y., Jiao F., Qiu Y., Li W., Lao F., Zhou G., et al. 2009 .The effect of [email protected](OH)22 nanoparticles on the release of Th1/Th2 cytokines and induction of TNF-alpha mediated cellular immunity. Biomaterials. 30(23-24):3934–3945.

Liu Y., Wang L., Zhang Y., Zhang W., Chen X., Yang T., et al. 2014. Uniform-sized water-in-oil vaccine formulations enhance immune response against Newcastle disease and avian influenza in chickens. Int. Immunopharmacol. 2014;23(2):603–608. 10.1016/j.intimp.2014.10.011

Lu T., Hu F., Yue H., Yang T., Ma G. 2020. The incorporation of cationic property and immunopotentiator in poly (lactic acid) microparticles promoted the immune response against chronic hepatitis B. J. Control. Release. 321:576–588. 10.1016/j.jconrel.2020.02.039

Ma X., Bi S., Wang Y., Chi X., Hu S. 2019.Combined adjuvant effect of ginseng stem-leaf saponins and selenium on immune responses to a live bivalent vaccine of Newcastle disease virus and infectious bronchitis virus in chickens. Poult Sci. 98(9):3548–3556. 10.3382/ps/pez207

Park H., Wang D., Hong H., Shin K. 2017. Antitumor and antimetastatic activities of pectic polysaccharides isolated from persimmon leaves mediated by enhanced natural killer cell activity. J. Funct. Foods. 37:460–466. 10.1016/j.jff.2017.08.027

Ren L., Zhang J., Zhang T. 2021. Immunomodulatory activities of polysaccharides from Ganoderma on immune effector cells. Food Chem. 340:127933. 10.1016/j.foodchem.2020.127933

Su C., Duan X., Liang L., Feng W., Zheng J., Fu X., et al. 2014. Lycium barbarum polysaccharides as an adjuvant for recombinant vaccine through enhancement of humoral immunity by activating Tfh cells. Vet. Immunol. Immunopathol. 158(1-2):98–104. 10.1016/j.vetimm.2013.05.006

Sun B., Yu S., Zhao D., Guo S., Wang X., Zhao K. 2018. Polysaccharides as vaccine adjuvants. Vaccine. 36(35):5226–5234. 10.1016/j.vaccine.2018.07.040

Sun H., Fei L., Zhu B., Shi M. 2020. Quick and improved immune responses to inactivated H9N2 avian influenza vaccine by purified active fraction of Albizia julibrissin saponins. BMC Vet Res. 16(1):427. 10.1186/s12917-020-02648-1

Tang C., Sun J., Liu J., Jin C., Wu X., Zhang X., et al. 2019. Immune-enhancing effects of polysaccharides from purple sweet potato. Int. J. Biol. Macromol. 123:923–930. 10.1016/j.ijbiomac.2018.11.187

Wang J., Ge B., Li Z., Guan F., Li F. 2016. Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis. Carbohydr. Polym. 140:6-12. 10.1016/j.carbpol.2015.12.050

Wang Q., Meng X., Zhu L., Xu Y., Cui W., He X., et al. 2019. A polysaccharide found in Paulownia fortunei flowers can enhance cellular and humoral immunity in chickens. Int. J. Biol. Macromol. 130:213–219. 10.1016/j.ijbiomac.2019.01.168

Wang M., Meng X., Yang R., Qin T., Li Y., Zhang L., et al. 2013. Cordyceps militaris polysaccharides can improve the immune efficacy of Newcastle disease vaccine in chicken. Int. J. Biol. Macromol. 59:178–183. 10.1016/j.ijbiomac.2013.04.007

Wu Y., Wei W., Zhou M., Wang Y., Wu J., Ma G., et al. 2012. Thermal-sensitive hydrogel as adjuvant-free vaccine delivery system for H5N1 intranasal immunization. Biomaterials. 33(7):2351–2360. 10.1016/j.biomaterials.2011.11.068

Xie F., Li Y., Su F., Hu S. 2012. Adjuvant effect of Atractylodis macrocephalae Koidz polysaccharides on the immune response to foot-and-mouth disease vaccine. Carbohydr. Polym. 87(2):1713–1719. 10.1016/j.carbpol.2011.09.080

Xie X., Ma L., Zhou Y., Shen W., Xu D., Dou J., et al. 2019. Polysaccharide enhanced NK cell cytotoxicity against pancreatic cancer via TLR4/MAPKs/NF-kappaB pathway in vitro/vivo. Carbohydr. Polym. 225:115223. 10.1016/j.carbpol.2019.115223

Xu W., Fang S., Cui X., Guan R., Wang Y., Shi F., et al. 2019. Signaling pathway underlying splenocytes activation by polysaccharides from Atractylodis macrocephalae Koidz. Mol. Immunol. 111:19–26. 10.1016/j.molimm.2019.03.004

Yang H., Zhang P., Xu X., Chen X., Liu Q., Jiang C. 2019. The enhanced immunological activity of Paulownia tomentosa flower polysaccharide on Newcastle disease vaccine in chicken. Biosci. Rep. 39(5): BSR20190224. 10.1042/BSR20190224

Yang L., Hu Y., Xue J., Wang F., Wang D., Kong X., et al. 2018. Compound Chinese herbal medicinal ingredients can enhance immune response and efficacy of RHD vaccine in rabbit. Vaccine. 26(35):4451–4455. 10.1016/j.vaccine.2008.06.075

Yuan L., Wang Y., Li Z., Ma X., Cui X., Chi X., et al. 2020. Sunflower seed oil containing ginseng stem-leaf saponins (E515-D) is a safe adjuvant for Newcastle disease vaccine. Poult. Sci. 99(10):4795–4803. 10.1016/j.psj.2020.06.063

Yang Y., Xing R., Liu S., Qin Y., Li K., Yu H., et al. 2020. Chitosan, hydroxypropyltrimethyl ammonium chloride chitosan and sulfated chitosan nanoparticles as adjuvants for inactivated Newcastle disease vaccine. Carbohydr. Polym. 229:115423. 10.1016/j.carbpol.2019.115423

Yu Y., Song Q., Huang L., Shen M., Yu Q., Chen Y., et al. 2020. Immunomodulatory activities of sulfated Cyclocarya paliurus polysaccharides with different degrees of substitution on mouse spleen lymphocytes. J. Funct. Foods. 64:103706. 10.1016/j.jff.2019.103706

Zeng P., Li J., Chen Y., Zhang L. 2019. The structures and biological functions of polysaccharides from traditional Chinese herbs. Progress in molecular biology and translational science. 163:423–444. 10.1016/bs.pmbts.2019.03.003

Zhai L., Li Y., Wang W., Hu S. 2011a. Enhancement of humoral immune responses to inactivated Newcastle disease and avian influenza vaccines by oral administration of ginseng stem-and-leaf saponins in chickens. Poult. Sci. 90(9):1955–1959. 10.3382/ps.2011-01433

Zhai L., Li Y., Wang W., Wang Y., Hu S. 2011b. Effect of oral administration of ginseng stem-and-leaf saponins (GSLS) on the immune responses to Newcastle disease vaccine in chickens. Vaccine. 29(31):5007–5014. 10.1016/j.vaccine.2011.04.097

Zhang W., Gong L., Liu Y., Zhou Z., Wan C., Xu J., et al. 2020. Immunoenhancement effect of crude polysaccharides of Helvella leucopus on cyclophosphamide-induced immunosuppressive mice. J. Funct. Foods. 69:103942. 10.1016/j.jff.2020.103942

Zhang W., Wang L., Liu Y., Chen X., Li J., Yang T., et al. 2014. Comparison of PLA microparticles and alum as adjuvants for H5N1 influenza split vaccine: adjuvanticity evaluation and preliminary action mode analysis. Pharm. Res. 31(4):1015–1031. 10.1007/s11095-013-1224-z

Zhao Y., Yan B., Wang Z., Li M., Zhao W. 2020. Natural polysaccharides with immunomodulatory activities. Mini. Rev. Med. Chem. 20(2):96–106. 10.2174/1389557519666190913151632