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We analyzed the relation between the assessment of grape berry skin thickness by means of histology sections and instrumental mechanical properties measurements. Berry skin of Vitis vinifera L. cultivar Corvina vineyards from Valpolicella Valpantena zone (Verona, Italy) were tested, evidencing a strong correlation between the two thickness determination methods. The middle or equatorial berry skin portion was found to be the less variable in instrumental skin thickness determination. In addition, unlike other studies, no correlation between the skin thickness and cell layers number was found.