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antioxidant activity, carbonyl groups, malondialdehyde, oxidation level, Ricotta cheese, shelf life


To extend the shelf life of buffalo Ricotta cheese, a process was assessed that included a second heat treatment followed by homogenisation and hot packaging. The microbiological and chemical characteristics as well as the oxidation degree of the product were determined over storage for 21 days using total antioxidant activity, redox potential, malondialdehyde content, and protein-bound carbonyl content. Homogenised buffalo Ricotta cheese has a longer shelf life than traditional Ricotta cheese, although the process could be optimised to reduce the total bacterial load during storage. No significant oxidative damage occurred during storage. This innovative process could promote the market expansion of Ricotta cheese.
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Addeo F. and Coppola S. 1983. Aspetti tecnologici e microbiologici della trasformazione del latte di bufala in mozzarella e ricotta. Il latte 8:706-723.

Andrei S., Bunea A., Pintea A., Matei S. and Cozma A.M. 2015. Comparative study of antioxidant activity in fresh and pasteurized goat milk. Bulletin UASVM Veterinary Medicine 72:336-340.

Association of Official Analytical Chemists international. 2000. 17thed. Official Methods of Analysis, Gaithersburg, MD, USA.

Augustyniak E., Adam A., Wojdyla K., Rogowska-Wrzesinska A., Willetts R., Korkmaz A., Atalay M., Weber D., Grune T., Borsa C., Gradinaru D., Bollineni R.C., Fedorova M. and Griffiths H.R. 2015. Validation of protein carbonyl measurement: A multi-centre study. Redox Biology 4:149-157. DOI:

Balestrieri M., Spagnuolo M.S., Cigliano L., Storti G., Ferrara L., Abrescia P. and Fedele E. 2002. Evaluation of oxidative damage in mozzarella cheese produced from bovine or water buffalo milk. Food chemistry 77(3):293-299. DOI:

Bergamo P., Fedele E., Balestrieri M., Abrescia P. and Ferrara L. 1998. Measurement of malondialdehyde levels in food by high-performance liquid chromatography with fluorometric detection. J. agr. food chem. 46(6):2171-2176.

Branciari R., Ranucci D., Trabalza-Marinucci M., Codini M., Orru M., Ortenzi R., Forte C., Ceccarini M.R. and Valiani A. 2014. Evaluation of antioxidant stability of Pecorino cheese made from raw milk of ewes fed Rosmarinus officinalis L. leaves. Intern. J. Food Sci. Tech., 50:558-65.

Caldeo V. 2015. Oxidation-reduction potential and its influence on Cheddar cheese quality. PhD Thesis, University College Cork.

Calligaris S., Manzocco L., Anese M. and Nicoli M.C. 2004. Effect of heat-treatment on the antioxidant and pro-oxidant activity in milk. Int. Dairy j. 14:421-427.

Casti D., Scarano C., Pala C., Cossu F., Lamon S., Spanu V., Ibba M., Mocci A.M., Tedde F., Nieddu G., Spanu C. and De Santis E.P.L. 2016. Evolution of the microbiological profile of vacuum-packed ricotta salata cheese during shelf-life. Ital. J. Food Safety 5:5501:57-60.

Cherchi A., Porcu M., Spanedda L. and Tuberoso C.I.G. 1999. Caratteristiche chimico-fisiche, organolettiche e microbiologiche di ricotta ovina prodotta in Sardegna. La Rivista diScienze dell’Alimentazione 28:307-314.

Citta A., Folda A., Scaloni V., Scutari G., Bindoli A., Bellamio M., Feller E. and Rigobello M.P. 2017. Oxidative changes in lipids, proteins, and antioxidants in yogurt during shelf life. Food Sci. Nutr. 5 (6):1079-1087

Dalsgaard T.K., Sørensen J., Bakman M., Vognsen L., Nebel C., Albrechtsen R. and Nielsen J.H. 2010. Light-induced protein and lipid oxidation in cheese: Dependence on fat content and packaging conditions. Dairy Sci. Technol. 90:565-577.

Farkye N.Y. 2004. Acid-and Acid/Rennet-curd Cheeses. Part C: Acid-heat coagulated Cheeses. In: Cheese. Chemistry, Physics and microbiology. Third Edition. Edited by Patrick F. Fox, Paul L.H. McSweeney, Timothy M. Cogan and Timothy P. Guinee pp. 343-348.

Fedele E. and Bergamo P. 2001. Protein and lipid oxidative stresses during cheese manufacture. J. Food Sci. 66(7):932-935. DOI:

Fenaille F., Parisod V., Visani P., Populaire S., Tabet J.-C. and Guy P. A. 2006. Modifications of milk constituents during processing: A preliminary benchmarking study. Int. Dairy J. 16:728-739. DOI:

Feng X., Li C., Ullah N., Cao J., Lan Y., Ge W., Hackman R.M., Li Z. and Chen L. 2015. Susceptibility of whey protein isolate to oxidation and changes in physicochemical, structural, and digestibility characteristics. J. Dairy Sci. 98(11):7602-7613. DOI:

FIL-IDF 172: 2001 / ISO 14156:2001-Milk and Milk products-extraction methods for lipids and liposoluble compounds.

FIL-IDF. 1991. Chemical methods for evaluating proteolysis in cheese maturation, No. 261. Brussels, Belgium: International Dairy Federation.

FIL-IDF. 1993. Milk. Determination of nitrogen content. Kjeldahl method; Standard 20B. Annex I: Modified procedure for milk products. Brussels; Belgium: International Dairy Federation.

Hough G., Puglieso M.L., Sanchez R. and Da Silva O.M. 1999. Sensory and microbiological shelf-life of a commercial Ricotta cheese. J. Dairy Sci. 82(3):454-459.

International Dairy Federation. 1986. Cheese and processed cheese products. Determination of dry matter. IDF Standard No. 4. FIL-IDF, Brussels, Belgium.

International Dairy Federation. 1988. Determination of dry salt content. IDF Standard No. 12B. FIL-IDF, Brussels, Belgium.

Katalinic V., Milos M., Kulisic T. and Jukic M. 2006. Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chemistry 94:550-557. DOI:

Khan I.T., Nadeem M., Imram M., Ajmal M. and Ali S. 2018. Antioxidant activity, fatty acids characterization and oxidative stability of Gouda cheese fortified with mango (Mangifera indicaL.) kernel fat. J. Food Sci. Technol. 155:992-1002.

Korpela R., Ahotupa M., Korhonen H. and Syvaoja E.L. 1995. Antioxidant properties of cow’s milk. In: Proceedings of the NJF/NMR, Seminar n. 252, pp. 157-159, Turku, Finland.

Lee N.K., Jeewanthi R.K.C., Park E.H. and Paik H.D. 2016. Short communication: Physicochemical and antioxidant properties of Cheddar-type cheese fortified with Inula Britannicaextract. J. Dairy Sci. 99:83-88. DOI:

Lindmark-Mansson H. and Akesson B. 2000. Antioxidant factors in milk. British Journal of Nutrition, 84 (1):103-110. DOI:

Lipan L., Sánchez-Rodríguez L., Cano-Lamadrid M., Collado-González J., Noguera-Artiaga L., Sendra E. and Carbonell-Barrachina A.A. 2017. Modernization of traditional food processes and products. In: Consumer trends and new product opportunities in the food sector. Editedby Klaus G. Grunert. pp. 113-133. DOI:

Mancuso I., Cardamone C., Fiorenza G., Macaluso G., Arcuri L., Miraglia V. and Scatassa M.L. 2014. Sensory and microbiological evaluation of traditional ovine ricotta cheese in modified atmosphere packaging. Ital. J. Food Safety 3(2):1725-1727. DOI:

Maubois J.L. and Kosikowski F.V. 1978. Making Ricotta cheese by ultrafiltration principles. J. Dairy Sci. 61:881-884.

Modler H.W. and Emmons D.B. 1989. Production and yield of whole milk Ricotta manufactured by a continuous process. II. Results and discussion. Milchwissenschaft 44:673-676.

Mortensen G., Bertelsen G., Mortensen B.K. and Stapelfeldt H. 2004. Light-induced changes in packaged cheeses -a review. Int. Dairy J. 14:85102. DOI:

Mucchetti G., Carminati D. and Pirisi A. 2002. Ricotta fresca vaccina ed ovina: osservazioni sulle tecniche di produzione e sul prodotto. Latte 27:154-166.

Mucchetti G. and Neviani E. 2006. Microbiologia e tecnologia lattiero-casearia. Tecniche Nuove (ed), Milano, IT.

Mucchetti G., Pugliese A. and Paciulli M. 2017. Characteristics of some important Itaian cheeses -Parmigiano Reggiano, Grana Padano, Mozzarella, Mascarpone and Ricotta. In: Cruz RMS, Veira MC (eds) Mediterranean foods. CRT Press, London.

Nikolova M., Evstatieva L. and Nguyen T.D. 2011. Screening of plant extracts for antioxidant properties. Botanica Serbica 35 (1):43-48.

Ottogalli G., Rondinini G. and Cappelletti C. 1981. Ricerche su alcuni aspetti microbiologici ed igienici delle ricotte. Annali di Microbiologia ed Enzimologia 31:77-87.

Palmas F., Carta A., Cosentino S., Fadda M.E., Gilberto G. and Mulargia A. F. 1994. Nuova tecnologia per la produzione di ricotta ovina: caratteristiche e valutazionedella conservabilità. La Rivista diScienze dell’Alimentazione 23:467-472.

Papastergiadis A., Mubiru E., Van Langenhove H. and De Meulenaer B. 2012. Malondialdehyde measurement in oxidized food: evaluation of the spectrophotometric thiobarbituric acid reactive substance (TBARS) test in various food. J. Agric. Food Chem. 60:9589-9594. DOI:

Pintado M.E and Malcata F.X. 2000. Characterization of whey cheese packaged under vacuum. J. Food Protection 63(2):216-221.DOI:

Pintado M.E., Macedo A.C. and Malcata F.X. 2001. Review: technology, chemistry and microbiology of whey cheeses. Food Sci. Technology Intern. 7:105-116.

Raharjo S. and Sofos J. N. 1993. Methodology for measuring malonaldehyde as a product of lipid peroxidation in muscle tissues: A review. Meat Science 35(2):145-169. DOI:

Raia G., Fedele E., Balestrieri M. and Bergamo P. 1996. Specific and sensitive HPLC method for malondialdehyde measurement in milk and cheese. In Food and Health: role of animal products. pp. 205-209 (Enne G. and Greppi G.F. eds.) Elsevier press, Amsterdam.

Reznick A.Z. and Packer L. 1994. Oxidative damage to proteins: spectrophotometric method for carbonyl assay. In Methods in enzymology (Vol. 233, pp. 357-363) Academic Press.

Salvadori Del Prato O. 2001. Trattato di Tecnologia Casearia. Calderini Edagricole, Bologna, IT.

Salvatore E., Pes M., Falchi G., Pagnozzi D., Furesi S., Fiori M., Addis M.F. and Pirisi A. 2014. Effect of whey concentration on protein recovery in fresh ovine ricotta cheese. J. Dairy Sci. 97:4686-4694. DOI:

Scaloni A., Perillo V., Franco P., Fedele E., Froio R., Ferrara L. and Bergamo P. 2002. Characterization of heat-induced lactosylation products in caseins by immunoenzymatic and mass spectrometric methodologies. Biochim. Biophys. Acta 1598:30-39.

Scarano C., Spanu C., Mocci A.M., Piras F., Demontis M., Murittu G., Pinna G., Santoru A., and De Santis E.P.L. 2019. Microbiological and physicochemical properties of smoked ricotta cheese during refrigeration and temperature abuse storage. Ital. J. Food Safety 8:8009, 102-109. DOI:

Segat A., Biasutti M., Iacumin L., Comi G., Baruzzi F., Carboni C. and Innocente N. 2013. Use of ozone in production chain of high moisture Mozzarella cheese. Food Sci. Tech. 55:513-520. DOI:

Shahani K.M. 1979. Newer techniques for making and utilization of Ricotta cheese. Proc. 1st Biennal Marschall International Cheese Conference, Madison, WI, 77-87.

Smithers G.W. 2008. Whey and whey protein –From “gutter-to-gold”. Int. Dairy J. 18(7):695-704. DOI:

Tango M.S.A. and Ghaly A.E. 1999. Amelioration of lactic acid production from cheese whey using micro-aeration. Biomass and Bioenergy 17:221-238.

Taticchi A., Bartocci S., Servili M., Di Giovanni S., Pauselli M., Mourvaki E., Meo Zilio D. and Terramoccia S. 2017. Effect on quanti-quality milk and mozzarella cheese characteristics with further increasing the level of dried stoned olive pomace in diet for lactating buffalo. Asian-Australas. J. Anim Sci. 30:1605-1611. DOI:

True L.C. 1973. Effect of various processing conditions on the yield of whey Ricotta cheese. Proc. 10th Marschall Invitational Cheese Seminar, Madison, WI, 1-11.

Unal G. 2012. Antioxidant activity of commercial dairy products. Agro FOOD Industry hi-tech 23(1):39-42.

Weatherup W. 1986. The effect of processing variables on the yield and quality of Ricotta. Dairy Ind. Int. 5:41-45.

Wilbey R.A., Scott J.E., Robinson R.K. 2012. Cheesemaking practice. Springer Science & Business Media.DOI: