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black raspberry, anthocyanin, flavonoid, polyphenol, pharmacokinetic


Black raspberry (BR, Rubus occidentalis) is a berry originating from North America. It has a high anthocyanin and flavonoid content that seems to be dependent on ripening. Therefore, the bioactive and pharmacokinetic effects of BR were evaluated using pre-matured BR(PBR) collected from May to June. The total polyphenol, flavonoid and vitamin C content in PBR decreased while anthocyanin increased from 0.13 to 10.70 mg/g at 35 days after fruit set. The antioxidant activities due to DPPH and ABTS determination decreased as BRs were matured. Moreover, it is clear that the content of many phenolic compounds including gallic acid, caffeic acid, p-coumaric acid, ferulic acid, rutin, myricetin, luteolin and kaempferol was diminished during maturing. Both ferulic acid and rutin of BR generated the highest decrease as compared to other phenolic compounds. The pharmacokinetic Tmax and Cmax of PBR were 0.6 h and 0.264 µg/mL, respectively. Based on these results, PBR can generate qualified functionality as food and/or as a raw medicinal material.

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Baek B.K., Lim C.W., Jung J.M., Choi M.J., Lee S.I., Lee H.K. and Jeon B.H. 2005. Effects of black raspberry wine on testosterone level of New Zealand white rabbits. Korean J. Orient. Physiol. Pathol. 19:375.

Bajcz A.W. 2014. Reproductive ecophysiology of Rubus occidentalis in southeastern Michigan I. A survey of tolerance ranges. Rhodora. 116:249.

Bala I., Bhardwaj V., Hariharan S., Kharade S.V., Roy N. and Ravi Kumar M.N. 2006. Sustained-release nanoparticulate formulation containing antioxidant-ellagic acid as potential prophylaxis system for oral administration. J. Drug Target. 14:27.

Bobinaite R., Viskelis P. and Venskutonis P.R. 2012. Variation of total phenolics, anthocyanins, ellagic acid and radical scavenging capacity in various raspberry (Rubus spp.) cultivars. Food Chem. 132:1495.

Borges G., Degeneve A., Mullen W. and Crozier A. 2010. Identification of flavonoid and phenolic antioxidants in black currants, blueberries, raspberries, red currants and cranberries. J. Agric. Food Chem. 58:3901.

Brand-Williams W., Cuvelier M.E. and Berset C. 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci. Technol. 28:25.

Chae K.S., Son R.H., Park S.Y., Kim K.A., Lee T.B. and Kwon J.W. 2014. Analytical method validation of ellagic acid as marker compound for standardization of black raspberry extract as functional ingredient. Food Eng. Prog. 18:1.

Chaovanalikit A. and Wrolstad R.E. 2004. Total anthocyanins and total phenolics of fresh and processed cherries and their antioxidant properties. J. Food Sci. 69:FCT67.

Chirinos R., Campos D., Arbizu C., Rogez H., Rees J-F., Larondelle Y., Noratto G. and Cisneros-Zevallos, L. 2007. Effect of genotype, maturity stage and post-harvest storage on phenolic compounds, carotenoid content and antioxidant capacity of Andean mashua tubers. J. Sci. Food Agric. 87:437.

Cho Y.J., Chun S.S., Kwon H.J., Kim J.H., Yoon S.J. and Lee K.H. 2005. Comparison of physiological activities between hot-water and ethanol extract of Bokbunja (Rubus coreanum F.). J. Korean Soc. Food Sci. Nutr. 34:790.

Chung B-Y., Lee K-S., Kim M-K., Choi Y-H., Kim M-K. and Cho J-Y. 2008. Physico-chemical properties of rainfall interception culture and open field culture soils of Rubus sp. in Gochang-gun, Jeollabuk-do. Korean J. Soil Sci. Fert. 41:303.

Duncan F.J., Martin J.R., Wulff B.C., Stoner G.D., Tober K.L., Oberyszyn T.M., Kusewitt D.F. and Van Buskirk A.M. 2009. Topical treatment with black raspberry extract reduces cutaneous UVB-induced carcinogenesis and inflammation. Cancer Prev. Res. 2:665.

Floegel A., Kim D.O., Chung S.J., Koo S.I. and Chun O.K. 2011. Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. J. Food Comp. Anal. 24:1043.

Jeong J.H., Jung H., Lee S.R., Lee H.J., Hwang K.T. and Kim T.Y. 2010. Anti-oxidant, anti-proliferative and anti-inflammatory activities of the extracts from black raspberry fruits and wine. Food Chem. 123:338.

Kähkönen M.P. and Heinonen M. 2003. Antioxidant activity of anthocyanins and their aglycons. J. Agric. Food Chem. 51:628.

Kampfenkel K., Montagu M.V. and Inze D.1995. Extraction and determination of ascorbate and dehydroascorbate from plant tissue. Anal. Biochem. 225:165.

KMA-Korea Meteorological Administration. 2017. Weather information.

Kang D-R., Chung Y-H., Shim K-S. and ShinD. 2015. Comparison of chemical properties and phenolic compound for ethanol extract of blueberry, bokbunja and mulberry and their pomace. Korean J. Organic Agri. 23:535.

Lee J., Dossett M. and Finn C.E. 2013. Anthocyanin fingerprinting of true bokbunja (Rubus coreanus Miq.) fruit. J. Funct. Foods. 5:1985.

Lee J., Dossett M. and Finn C.E.2014. Mistaken Identify: Clarification of Rubus coreanus Miquel (Bokbunja). Molecules. 19:10524.

Lee K.I., Kim S.M., Kim S.M. and Pyo B.S. 2011. Comparison of fatty acids and antibacterial activity against pathogen of acne in different parts of ripened black raspberry (Rubus coreanus Miquel). J. Korean Soc. Food Sci. Nutr. 40:466.

Lei F., Xing D.M., Xiang L., Zhao Y.N., Wang W., Zhang L.J. and Du L.J. 2003. Pharmacokinetic study of ellagic acid in rat after oral administration of pomegranate leaf extract. J. Chromatogr. B. 796:189.

Liu Z., Schwimer J., Liu D., Greenway F.L., Anthony C.T. and Wlotering EA. 2005. Black raspberry extract and fractions contain angiogenesis inhibitors. J. Agric. Food Chem. 53:3909.

Meda A., Lamien C.E., Romito M., Millogo J. and Nacoulma O.G. 2005. Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chem. 91:571.

Ogawa K., Sakakibara H., Iwata R., Ishii T., Sato T., Goda T., Shimoi K. and Kumazawa S. 2008. Anthocyanin composition and antioxidant activity of the crowberry (Empetrum nigrum) and other berries. J. Agric. Food Chem. 56:4457.

Ozgen M., Wyzgoski F.J., Tulio A.Z., Gazula A., Miller A.R., Scheerens J.C., Reese R.N. and Wright S.R. 2008. Antioxidant capacity and phenolic antioxidants of Midwestern black raspberries grown for direct markets are influenced by production site. HortScience. 43:2039.

Re R., Pellegrini N., Proteggente A., Pannala A., Yang M. and Rice-Evans C.1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26:1231.

Seeram N.P., Lee R. and Heber D. 2004. Bioavailability of ellagic acid in human plasma after consumption of ellagitannins from pomegranate (Punica granatum L.) juice. Clin. Chim. Acta. 348:63.

Singleton V.L. and Rossi Jr, J.A. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic. 16:144.

Smart R.C., Huang M-T., Chang R.L., Sayer J.M., Jerina, D.M. and Conney A.H. 1986. Disposition of the naturally occurring antimutagenic plant phenol, ellagic acid, and its synthetic derivatives, 3-O-decylellagic acid and 3,3’-di-O-methylellagic acid in mice. Carcinogenesis. 7:1663.

Tian Q., Giusti M.M., Stoner G.D. and Schwartz S.J. 2006. Characterization of a new anthocyanin in black raspberries (Rubus occidentalis) by liquid chromatography electrospray ionization tandem mass spectrometry. Food Chem. 94:465.

Torre L.C. and Barritt B.H. 1977. Quantitative evaluation of Rubus fruit anthocyanin pigments. J. Food Sci. 42:488.

Tulio Jr A.Z., Reese R.N., Wyzgoski F.J., Rinaldi P.L., Fu R., Scheerens J.C. and Miller A.R. 2008. Cyanidin 3-Rutinoside and cyaniding 3-Xylosylrutinoside as primary phenolic antioxidants in black raspberry. J. Agric. Food Chem. 56:1880.

Zhao C., Kim H.K., Kim S.Z., Chae H.J., Cui W.S., Lee S.W., Jeon J.H. and Park J.K. 2011. What is the role of unripe Rubus coreanus extract on penile erection? Phytother. Res. 25:1046.