Total polyphenol content and antioxidant properties of Solina (Triticum aestivum L.) and derivatives thereof

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Alessandra Durazzo
Gaetana Casale
Valentina Melini
Giuseppe Maiani
Rita Acquistucci


antioxidants, bread, soft wheat Solina, Total Polyphenol Content (TPC), traditional foods, whole wheat flours


This study aims to characterize grains and derivatives of Solina, an Italian traditional winter soft wheat (Triticum aestivum L.). Total polyphenol content and antioxidant properties were investigated in grains, whole flours and bread, both in aqueous-organic extracts and residues. Results showed the important contribution of hydrolysable polyphenols, isolated in the residue of the aqueous-organic extract, to antioxidant properties (over 90%  in grains and derivatives), and the key role played by milling and baking in antioxidant properties. Moreover, the analysis of potentially bioactive antioxidants remaining in the residues showed to be required for a comprehensive determination of antioxidant capacity in cereals. The study highlights Solina represents a valuable Italian traditional wheat cultivar with proved antioxidant properties.
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American Association of Cereal Chemists. 2003. Approved Methods of the AACC, 10th edn. Methods 55-31, St. Paul, MN, The Association.

Abdel-Aal E.M. and Rabalski I. 2013. Effect of baking on free and bound phenolic acids in wholegrain bakery products. Journal of Cereal Science 3:312.

Adom K.K., Sorrells M.E. and Liu R.H. 2003. Phytochemical profiles and antioxidant activity of wheat varieties. Journal of Agricultural and Food Chemistry 51:7825.

Arvola A., Lähteenmäki L., Dean M., Vassallo M., Winkelmann M., Claupein E., Saba A. and Shepherd R. 2007. Consumers’ beliefs about whole and refined grain products in UK, Italy and Finland. Journal of Cereal Science 46:197.
Aune D., Norat T., Romundstad P. and Vatten L.J. 2013. Whole grain and refined grain consumption and the risk of type 2 diabetes: a systematic review and dose-response meta-analysis of cohort studies. European Journal of Epidemiology 28: 845.
Benzie I.F.F. and Strain J.J. 1996. The ferric reducing ability of plasma (FRAP) as a measure of ‘‘antioxidant power’’: the assay. Analytical Biochemistry 239:70.

Carcea M., Durazzo A., Raguzzini A., Azzini E., Foddai M.S. and Maiani G. 2009. Bioactive components in wheat grains and their products. International Wheat Quality Conference-IV, Wheat science dynamics: challenges & opportunites, Saskatoon, 2-6 giugno 2009. Agrobios (International), 437-448.

Chandrasekara A. and Shahidi F. 2010. Content of insoluble bound phenolics in millets and their contribution to antioxidant capacity. Journal of Agricultural and Food Chemistry 58: 6706.

Connolly M.L., Tuohy, K.M. and Lovegrove, J.A. 2012. Wholegrain oat-based cereals have prebiotic potential and low glycaemic index. British Journal of Nutrition 108: 2198.
Duodu, K.G. 2011. Effects of Processing on Antioxidant Phenolics of Cereal and Legume Grains. In: Advances in Cereal Science: Implications to Food Processing and Health Promotion. Chapter 3, 31–54, 2011 American Chemical Society.

Durazzo A., Turfani V., Azzini E., Maiani G. and Carcea, M. 2013. Phenols, lignans and antioxidant properties of legume and sweet chestnut flours. Food Chemistry 140:666.

Hemery Y., Rouau X., Lullien-Pellerin V., Barron C. and Abecassis J. 2007. Dry processes to develop wheat fractions and products with enhanced nutritional quality. Journal of Cereal Science 46:327.

Hirawan R., Ser W.Y., Arntfirld S.D. and Beta T. 2010. Antioxidant properties of commercial, regular- and whole-wheat spaghetti. Food Chemistry 119: 258.

International Association for Cereal Science and Technology. 2003. Standard methods of ICC. Methods No. 104/1, 105/2, 110/1. Vienna, Austria: The Association .

Jensen S., Ostdal H., Skibsted L.H. and Thybo A.K. 2011. Antioxidants and shelf life of whole wheat bread. Journal of Cereal Science 53: 291.
Liyana-Pathirana C.M. and Shahidi F. 2007. The antioxidant potential of milling fractions from bread wheat and durum. Journal of Cereal Science 45:238.
Liu R.H. 2007. Whole grain phytochemicals and health. Journal of Cereal Science 46:207.
Manzocco L., Calligaris S., Mastrocola D., Nicoli M.C. and Lerici C.R. 2001. Review of non-enzymatic browning and antioxidant capacity in processed foods. Trends in Food Science and Technology 11: 340.

Mellen P., Walsh T. and Herrington D. 2008. Whole grain intake and cardiovascular disease. A meta-analysis. Nutrition Metabolism Cardiovascular Diseases 18:283.
Okarter N. and Liu R.H. 2010. Health benefits of whole grain phytochemicals. Critical reviews in Food Science and Nutritional 50:193.
Perez-Jimenez J. and Saura-Calixto F. 2005. Literature data may underestimate the actual antioxidant capacity of cereals. Journal of Agricultural and Food Chemistry 53:5036.

Pérez-Jiménez J. and Torres J.L. 2011. Analysis of non-extractable phenolic compounds in foods: the current state of the art. Journal of Agricultural and Food Chemistry 59(24): 12713.

Pérez-Jiménez J., Diaz-Rubio M.E., Saura-Calixto F. 2013. Non-extractable polyphenols, a major dietary antioxidant: occurrence, metabolic fate and health effects. Nutrition Research Reviews 26: 118.

Pomeranz Y. and Williams P.C. 1990. Wheat hardness: Its genetic,structural, and biochemical background, measurement, and significance. Pages 471-548 in: Advances in Cereal Science and Technology,Vol. X. Y. Pomeran,, ed. AACC International: St. Paul, MN.

Porfiri O., Torricelli R., Silveri D.D., Papa R., Barbaccia G. and Negri V. 2001. The Triticeae genetic resources of central Italy: collection, evaluation and conservation. Hereditas 135:187.

Pulido R., Bravo L. and Saura-Calixto F. 2000. Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. Journal of Agricultural and Food Chemistry 48: 3396.

Ragaee S., Seetharaman K. and Abdel-Aal el-SM. 2014. The impact of milling and thermal processing on phenolic compounds in cereal grains. Critical Reviews in Food Science and Nutrition 54:837.
Schatzkin A., Mouw T., Park Y., Subar A.F., Kipnis V., Hollenbeck A., Leitzmann M.F. and Thompson F.E. 2007. Dietary fiber and whole-grain consumption in relation to colorectal cancer in the NIH-AARP Diet and Health Study. American Journal of Clinical Nutrition 85:1353.
Saura-Calixto F. 2011. Dietary fibre as a carrier of dietary antioxidants: an essential physiological function. Journal of Agricultural and Food Chemistry 59:43-49.

Saura-Calixto F. 2012. Concept and health-related properties of nonextractable polyphenols: the missing dietary polyphenols. Journal of Agricultural and Food Chemistry 60:11195.

Singleton V. L., Orthofer, R. and Lamuela-Raventos R. M. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin- Ciocalteau reagent. Methods in Enzymology 299: 152.

Slavin J.L., Jacobs D. and Marquart L. 2000. Grain processing and nutrition. Critical Reviews in Food Science and Nutrition 40:309.

Vitaglione P., Napolitano A. and Fogliano V. 2008. Cereal dietary fibre: a natural functional ingredient to deliver phenolic compounds into the gut. Trends Foods Science and Technology 19:451.

Williams P.G., Grafenauer S.J. and O’Shea J.E. 2008. Cereal grains, legumes and weight management: a comprehensive review of the scientific evidence. Nutrition Reviews 66:171.