Identification of guaiacol producing Alicyclobacillus recovered from commercial orange juices distributed in Greek markets

Main Article Content

Patra Sourri
Agnieszka Dekowska
Joanna Joanna Bucka-Kolendo
George-John Nychas
Chrysoula Tassou
Agapi I Doulgeraki

Keywords

Abstract

The objective of this work was the isolation and characterization of Alicyclobacillus acidoterrestris strains from orange juice. In all, 72 isolates were recovered from commercial orange juice bought from the Greek market. The isolates were subjected to polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) to discriminate them at strain level and A. acidoterrestris types I and II. The restriction patterns of 16S rRNA gene revealed that isolates were categorized in different groups depending on the restriction endonucleases. Moreover, the digestion of the part of vdc operon gene with HphI restriction enzyme successfully differentiated between A. acidoterrestris types I and II. In conclusion, RFLP succeeded in differentiating A. acidoterrestris isolates at strain and type levels.

Abstract 76 | PDF Downloads 51 XML Downloads 4 HTML Downloads 0

References

Cacho, P., Danyluk, M. and Rouseff, R. 2011. GC–MS quantification and sensory thresholds of guaiacol in orange juice and its correlation with Alicyclobacillus spp. Food Chem. 129: 45–50.

Chen, S., Tang, Q., Zhang, X., Zhao, G., Hu, X., Liao, X., Chen, F., Wu, J. and Xiang, H. 2006. Isolation and characterization of thermo-acidophilic endospore-forming bacteria from the concentrated apple juice-processing environment. Food Microbiol. 23: 439–445.

Dekowska, A., Niezgoda, J. and Sokolowska, B. 2018. Genetic Heterogeneity of Alicyclobacillus strains Revealed by RFLP Analysis of vdc Region and rpoB Gene. Biomed Res. Int. ID 9608756.

Doerge, D.R., Divi, R.L. and Churchwell, M.I. 1997. Identification of the Colored Guaiacol Oxidation Product Produced by Peroxidases. Anal. Biochem. 250: 10–17.
Eguchi, S., Manfio, G., Pinhatti, M., Azuma, E. and Variane, S. 1999. Acidothermophilic Sporeforming Bacteria (ATSB) in Orange Juices: Detection Methods, Ecology, and Involvement in the Deterioration of Fruit Juices. Braz. Assoc. Citrus Export. 25–35.
Eiroa, M.N.U., Junqueira, V.C.A. and Schmidt, F.L.1999. Alicyclobacillus in Orange Juice: Occurrence and Heat Resistance of Spores. J. Food Prot. 62: 883–886.

Gocmen, D., Elston, A., Williams, T., Parish, M. and Rouseff, R. 2005. Identification of medicinal off-flavours generated by Alicyclobacillus species in orange juice using GC-olfactometry and GC-MS. Lett. Appl. Microbiol. 40: 172–177.

Gouws, P.A., Gie, L., Pretorius, A. and Dhansay, N. 2005. Isolation and identification of Alicyclobacillus acidocaldarius by 16S rDNA from mango juice and concentrate. Int. J. Food Sci. Technol. 40; 789–792.

Griffiths, M. 2000. The New Face of Food Born Illness. Can. Meat. Sci. Assoc. 1: 6–9.

Hartyáni, P., Dalmadi, I. and Knorr, D. 2013. Electronic nose investigation of Alicyclobacillus acidoterrestris inoculated apple and orange juice treated by high hydrostatic pressure. Food Control 32; 262–269.

IFU Method No 12, 2004/2007. International Federation of Fruit Juice Producers, Method on the Detection of Taint Producing Alicyclobacillus in Fruit Juices. IFU: Copenhagen, Denmark, 2007.

Klijn N., Weerkamp A.H. and de Vos W.M. 1991. Identification of mesophilic lactic acid bacteria by using polymerase chain reaction-amplified variable regions of 16S rRNA and specific DNA probes. Appl. Environ. Microbiol. 57 :3390–3393.

Lusardi, C., Previdi, M.P., Colla, F., Barbieri, G. and Bolzoni, L. 2000. Ability of Alicyclobacillus Strains to Spoil Fruit Juices and Nectars. Ind. Conserve Italy. 75: 151–161.

McKnight, I., Eiroa, M.; Sant’Ana, A. and Massaguer, P. 2015. Alicyclobacillus acidoterrestris in pasteurized exotic Brazilian fruit juices: Isolation, genotypic characterization and heat resistance. Food Microbiol. 27: 1016–1022.
Molva, C., and Baysal, A.H. 2015. Evaluation of bioactivity of pomegranate fruit extract against Alicyclobacillus acidoterrestris DSM 3922 vegetative cells and spores in apple juice. LWT. 62: 989–995.

Osopale, B.A., Witthuhn, C.R., Albertyn, J. and Oguntoyinbo, F.A. 2016. Culture dependent and independent genomic identification of Alicyclobacillus species in contaminated commercial fruit juices. Food Microbiol. 56: 21–28.

Outbreak of Salmonella Serotype Muenchen Infections Associated with Unpasteurized Orange Juice—United States and Canada, June 1999. Available online: https://www.cdc.gov/mmwr/preview/mmwrhtml/mm4827a2.htm (accessed on 30 January 2022).

Pinhatti, M.E.M.C., Variane, S., Eguchi, S.Y. and Manfio, G.P. 1997. Detection of Acidothermophilic Bacilli in Industrialized Fruit Juices. Fruit Process. 9: 350–353.

Silva, F.V.M., Gibbs, P. and Silva, C.L.M. 2000. Establishing a new pasteurisation criterion based on Alicyclobacillus acidoterrestris spores for shelf-stable high-acidic fruit products. Fruit Proces 4: 138-141.

Silva, F. and Gibbs, P. 2001. Alicyclobacillus acidoterrestris spores in fruit products and design of pasteurization processes. Trends Food Sci. Technol. 12: 68–74.

Smit, Y., Cameron, M., Venter, P. and Witthuhn, R.C. 2011. Alicyclobacillus spoilage and isolation—A review. Food Microbiol. 28: 331–349.

Sourri, P., Doulgeraki, A.I., Tassou, C.C. and Nychas, G.-J.E. 2019. A single enzyme PCR-RFLP assay targeting V1-V3 region of 16S rRNA gene for direct identification of Alicyclobacillus acidoterrestris from other Alicyclobacillus species. J. Appl. Genet. 60: 225–229.

Sourri, P., Tassou, C.C., Nychas, G.-J.E. and Panagou, E.Z. 2022. Fruit Juice Spoilage by Alicyclobacillus: Detection and Control Methods—A Comprehensive Review. Foods. 11: 747.

Uchida, R. and Silva, F.V. 2017. Alicyclobacillus acidoterrestris spore inactivation by high pressure combined with mild heat: Modeling the effects of temperature and soluble solids. Food Control. 73: 426–432.

Van Luong, T.S., Moir, C.J., Kaur, M., Frank, D., Bowman, J.P. and Bradbury, M.I. 2019. Diversity and guaiacol production of Alicyclobacillus spp. from fruit juice and fruit-based beverages. Int. J. Food Microbiol. 2, 311:108314.

Vieira, M.C., Teixeira, A.A., Silva, F.M., Gaspar, N. and Silva, C.L.M. 2002. Alicyclobacillus acidoterrestris spores as a target for Cupuaçu (Theobroma grandiflorum) nectar thermal processing: kinetic parameters and experimental methods. Int. J. Food Microbiol. 77: 71–81.

Witthuhn, R.C., Smit, Y., Cameron, M. and Venter, P. 2011. Isolation of Alicyclobacillus and the influence of different growth parameters. Int. J. Food Microbiol. 146: 63–68.

Yamazaki, K., Teduka, H., Inoue, N. and Shinano, H. 1996. Specific primers for detection of Alicyclobacillus acidoterrestris by RT-PCR. Lett. Appl. Microbiol. 23: 350–354.

Zhang, J., Yue, T. and Yuan, Y. 2013. Alicyclobacillus contamination in the production line of kiwi products in China. PLoS One. 2,8(7):67704.