Some factors affecting the bioactive substances of lactogenic tea

Main Article Content

Sunisa Siripongvutikorn
Chanisara Kluabpet
Chaninat Anantapan
Vatcharee Seechamnanturakit


breastfeeding, galactagogue herbal tea, herbal tea brew, lactogenic product, particle size distribution, total phenolic and flavonoid content


This study aims to perform the phytochemical screening composition for galactagogue herbs and determining the total phenolic (TPC) and flavonoid contents (TFC). Terpenes, saponins, tannins, alkaloids, phenolics, and flavonoids were found in water extracts of banana inflorescence (Musa sapientum L), white holy basil leaves (Ocimum sanctum L.), rhizomes of Thai ginger (Zingiber officinale Roscoe), and powdered lactogenic product. The results show that the TPC were greater than the TFC for all fresh parts of banana inflorescences. Following the M1 method, fresh banana floret gave the greatest TPC, superior to those with M2 or M3, while the TFC with M1 and M2 methods applied to banana floret was significantly greater than that of banana bracts or banana cores. After harvest and dehydration of the banana inflorescence, TPC and TFC after one-day storage were higher than those at five-days of storage. Meanwhile, TPC and TFC on both fresh and dehydrated white holy basil tended to decrease due to heat intolerance, deterioration, and brewing. In contrast, TPC and TFC of Thai ginger increased on dehydration. The maceration with press-shear force interaction (PAI) pretreatment, and with PAI combined with moist heat, strongly increase the bioactive contents. SEM imaging demonstrated that PAI with moist heat tended to enlarge the micro-pore volume and simultaneously increased pores, resulting in more adsorption of these compounds.

Abstract 118 | PDF Downloads 152 HTML Downloads 49 XML Downloads 42


Amorim M., Hobby E., Zamora-Kapoor A., Perham-Hester K.A. and Cowan S.K. 2023. The heterogeneous associations of universal cash-payouts with breastfeeding initiation and continuation. SSM Popul. Health. 22: 101362.
Atraqchi N.H.A. and Hamed W.M.A.A.S. 2014. Preliminary phytochemical screening an in-vitro evaluation of antioxidant activity of Iraqi species of Silybum marianum seeds. Int. Res. J. Pharm. 5(5): 378–383.
Bajalan I, Zand M, Goodarzi M, Darabi M. 2017. Antioxidant activity and total phenolic and flavonoid content of the extract and chemical composition of the essential oil of Eremostachys laciniata collected from Zagros. Asian Pac J Trop Biomed 7(2): 144–146.
Budiono B., Pertami S.B., Kasiati, Arifah S.N. and Athoillah M.F. 2023. Lactogenic effect of Polyscias scutellaria extract to maintain postpartum prolactin and oxytocin in lactating rats. J Ayurveda Integr Med. 14: 100580.
Chandra S., Khan S., Avula B., Lata H., Yang M.H., ElSohly M.A., Khan I.A. 2014. Assessment of total phenolic and flavonoid content, antioxidant properties, and yield of aeroponically and conventionally grown leafy vegetables and fruit crops: a comparative study. J Evid-Based Compl Altern Med 2014: 253875.
Chan L.P., Chou T.H., Ding H.Y., Chen P.R., Chiang F.Y., Kuo P.L. and Liang C.H. 2012. Apigenin-induces apoptosis via tumor necrosis factor receptor- and BcL-2-mediated pathway and enhances susceptibility of head and neck squamous cell carcinoma to 5-fluorouracil and cisplatin. Biochim. Biophys. Acta 1820: 1081–1091.
Cheng F.S., Loong T.M., Cheng F.W., Lisa A M., Jacqueline J H. and Howe O.J. 2020. Oral Galactagogues (natural therapies or drugs) for increasing breast milk production in mothers of nonhospitalised term infants. CDSR 5(5): CD011505. https://doi. org/10.1002/14651858.CD011505.pub2
Chrzan-Detkoś M., Walczak-Kozlowska T. and Pietkiewicz A. 2021. Improvement of the breastfeeding self-efficacy and postpartum mental health after lactation consultations – Observational study. Midwifery 94: 102905.
Cortes-Macías E., Selma-Royo M., García-Mantrana I., Calatayud M., González S., Martínez-Costa C., et al. 2021. Maternal diet shapes the breast milk microbiota composition and diversity: impact of mode of delivery and antibiotic exposure. J Nutr. 151: 330–340.
El-Loly M.M. 2022. Review: Colostrum ingredients, its nutritional and health benefits – an overview. Clin Nutr. Open Science 44: 126–143.
Embleton N.D., Moltu S.J., Lapillonne A., H P van den Akker C., Carnielli V., Fusch C., et al. 2023. Enteral Nutrition in Preterm Infants (2022): A Position Paper From the ESPGHAN Committee on Nutrition and Invited Experts. J Pediatr Gastroenterol Nutr. 76(2): 248–268.
Fu X.B., Zhang J.Z., Guo L., Xu Y.G., Sun L.Y., Wang S.S., et al. 2014. Protective role of luteolin against cognitive dysfunction induced by chronic cerebral hypoperfusion in rats. Pharmacol. Biochem. Behav. 126: 122–130.
Guo C., Chen X.and Xiong P. 2014. Baicalin suppresses iron accumulation after substantia nigra injury: relationship between iron concentration and transferrin expression. Neural Regen. Res. 9: 630–636.
Huang Y., Tsang S.Y., Yao X., Chen Z.Y. 2005. Biological properties of baicalein in cardiovascular system. Curr. Drug Targets Cardiovasc. Haematol. Disord. 5: 177–184.
Jaiswal B.S. and Solanum T. 2012. A review of its traditional uses, phytochemistry and pharmacology. Int. J. Pharma Bio Sci. 3(3): 104–111.
Karcz K. and KrÓlak-Olejnik B. 2020. Vegan or vegetarian diet and breast milk composition – a systematic review. Crit Rev Food Sci Nutr. 61 (7): 1–18.
Kim Y.J. 2020. Pivotal roles of prolactin and other hormones in lactogenesis and the nutritional composition of human milk. CEP 63(8): 312–313.
Kong W.S., Tsuyama N., Inoue H., Guo Y., Mokuda S., Nobukiyo A., et al. 2021. Long-chain saturated fatty acids in breast milk are associated with the pathogenesis of atopic dermatitis via induction of inflammatory ILC3s. Scientific Reports 11: 13109.
Korus A. 2011. Effect of preliminary processing, method of drying and storage temperature on the level of antioxidants in kale (Brassica oleraceaL.var.acephala) leaves. LWT-Food Sci. Technol. 44: 1711–1716.
Kumar G.S., Jayaveera K.N., Kumar C.K., Sanjay U.P., Swamy B.M. and Kumar D.V. 2007. Antimicrobial effects of Indian medicinal plants against acne-inducing bacteria. Trop. J. Pharm. Res. 6(2): 717–723.
Lau B.F., Kong K.W., Leong K.H., Sun J., He X., Wang Z., et al. 2020. Banana inflorescence: Its bio-prospects as an ingredient for functional foods. Trends Food Sci Technol. 97: 14–28.
Lawrence R.A. 2022. 19 – Induced Lactation and Relactation (Including Nursing an Adopted Baby) and Cross-Nursing. Breastfeeding (Ninth Edition), A Guide for the Medical Profession. 628–645.
Li M., Li Q., Zheng Y., Shi X., Zhang J., Ma C., et al. 2020. New insights into the alterations of full spectrum amino acids in human colostrum and mature milk between different domains based on metabolomics. Eur. Food Res. Technol. 246(5): 1119– 1128.
Li M., Chen J., Shen X., Abdlla R., Liu, L., Yue X., et al. 2022. Metabolomics-based comparative study of breast colostrum and mature breast milk. Food Chem. 384: 132491.
Liang C.-H., Lin Y.-H., Lin Y.-K. and Chiang C.-F. 2023. Hair growth-promotion effects and antioxidant activity of the banana flower extract HappyAngel®: double-blind, placebo-controlled trial. Food Sci. Hum. Wellness 12: 1917–1923.
Luz C., Calpe J., Quiles J.M., Torrijos R. Vento M., Gormaz M., et al. 2021. Probiotic characterization of Lactobacillus strains isolated from breast milk and employment for the elaboration of a fermented milk product. J. Funct. Foods. 84: 104599.
Martín-Carrasco I., Carbonero-Aguilar P., Dahiri B., Moreno I.M. and Hinojosa M. 2023. Comparison between pollutants found in breast milk and infant formula in the last decade: A review. Sci Total Environ. 875: 162461.
McBride G.M., Stevenson R., Zizzo G., Rumbold A.R., Amir L.H., Keir A., et al. 2023. Women’s experiences with using domperidone as a galactagogue to increase breast milk supply: an Australian cross-sectional survey. Int. Breastfeed. J. 18(11): 1–9.
Miranda A.R., Scotta A.V., Cortez M.V., González-GarcÍa N., Galindo-VillardÓn and Soria E.A. 2022. Association of Dietary Intake of Polyphenols with an Adequate Nutritional Profile in Postpartum Women from Argentina. Prev Nutr Food Sci. 27(1): 20–36.
Naidu A.S. 2000. Lactoferrin, lactoperoxidase. In: Naidu AS (Ed.). Natural food antimicrobial systems. New York: CRC Press. pp. 17–132.
Nam S.Y., Jeong H.J. and Kim H.M. 2017. Kaempferol impedes IL-32-induced monocyte-macrophage differentiation. Chem. Biol. Interact. 274: 107–115.
Naveed M., Hejazi V., Abbas M., Kamboh A.A., Khan G.J., Shumzaid M., et al. 2018. Chlorogenic acid (CGA): A pharmacological review and call for further research. Biomed. Pharmacother. 97, 67–74.
Odeja O.O., Obi G., Ogwuche C.E., Elemike E.E. and Oderinlo O.O. 2014. Phytochemical screening, Antioxidant and Antimicrobial activities of Senna occidentalis (L.) leaves. Int. J. Herb. Med. 2(4): 26–30.
Olaimat A.N., Osaili T.M., Al-Holy M.A., Al-Nabulsi A.A., Obaid R.S., Alaboudi A.R., et al. 2020. Microbial safety of oily, low water activity food products: A review. Food Microbiol. 92: 103571.
Panyayong C. and Srikaeo K. 2022. Foods from banana inflores-ences and their antioxidant properties: An exploratory case in Thailand. Int. J. Gastron. Food Sci. 28: 100436.
Panyayong C. and Srikaeo K. 2023. Effects of hydrocolloids on the qualities of pureed banana inflorescences prepared for individuals with dysphagia. Food Hydrocolloids for Health 3: 100129.
Parekh J. and Chanda S. 2007. In vitro antimicrobial activity and phytochemical analysis of some Indian medicinal plants. Turkish J. Biol. 31(1): 53–58.
Perrella S., Gridneva Z., Lai C.T., Stinson L., George A., Bilstin-John S., et al. 2021. Human milk composition promotes optimal infant growth, development and health. Semin. Perinatol. 45: 151380.
Rudrakshi S., Surekha B. and Preetinder K. 2018. Effect of Drying Methods on Biochemical Quality of Basil Leaf. Agric. Res. J 55(2): 331-335.
Samal S., Sarumathy S., Priyadharshini A., Sudhir M., Juzer F., Karthikeswari A., et al. 2021. Safety and efficacy of Hemocare® LACT on breast milk production and prolactin levels in postpartum mothers: An open-label, placebo controlled, randomized trial. Phytomedicine Plus 1:100132.
Sharma K., Bhatnagar M. 2011. Asparagus racemosus (Shatavari): A
versatile female tonic. Int J Pharm Bio Arch 2: 855–863. Sinkiewicz-Darol E., Martysiak-Zurowska D., Puta M., Adamczyk I.,
Barbarska O., Wesolowska A., et al. 2022. Nutrients and Bioactive Components of Human Milk After One year of Lactation: Implication for Human Milk Banks. J Pediatr Gastroenterol Nutr. 74(2): 284–291.
Srinivas N.R. 2010. Baicalin, an emerging multi-therapeutic agent: pharmacodynamics, pharmacokinetics and considerations from drug development perpectives. Xenobiotica 40: 357–367.
Tabares F.P., Jaramillo J.V.B. and Ruiz-Cortés Z.T. 2014. Pharmacological overview of galactogogues. Vet Med Int. 2014: 602894.
TarragÓ T., Kichik N., Claasen B., Prades R., TeixidÓ M. and Giralt E. 2008. Baicalin, a prodrug able to reach the CNS, is a propyl oligo-peptidase inhibitor. Bioorg. Med. Chem. 16: 7516–7524.
Ukoha P.O., Cemaluk E.A., Nnamdi O.L., and Madus E.P. 2011. Tannins and other phytochemical of the Samanaea saman pods and their antimicrobial activities. Afr. J. Pure Appl. Chem. 5(8): 237–244.
Wang X., Zhao Y. and Zhong X. 2014. Protective effects of baicalin on decidua cells of LPS-induced mice abortion. J. Immunol. Res. 2014: 859812.
Wei X., Yang J., Wu C. 2006. Anxiolytic effect of baicalin in mice.
Asian J. Tradit. Med. 1: 3–4.
Wu S.M., Wu H.Y., Wu Y.J., Liu L., Cai R.P. and Xu Y.J. 2012. Effects
of Baicalin and Ligustrazine on airway inflammation and remodeling and underlying mechanism in asthmatic rats. Adv. Biosci. Biotechnol. 3: 585–591.
Xie H., Xu L., Niu H. and Cao Z. 2018. Particle sizing from Fraunhofer diffraction pattern using a digital micro-mirror device and a single photodiode. Powder Technol. 332:351–358.
Yadav M., Chatterji S., Gupta S.K., and Watal G. 2014. Preliminary phytochemical screening of six medicinal plants used in traditional medicine. Int. J. Pharm. Pharm. Sci. 6(5): 539–542.
Yang J., Yang X. and Li M. 2012. Baicalin, a natural compound, promotes regulatory T cell differentiation. BMC Complement. Altern. Med. 12: 64.
Yang X., Yang J. and Zou H. 2013. Baicalin, inhibits IL-17-mediated joint inflammation in murine adjuvant-induced arthritis. Clin. Dev. Immunol. 2013: 268065.
Yang S., Fu Y., Wu X., Zhou Z., Xu J., Zeng X., Kuang N., Zeng Y. 2014. Baicalin prevents Candida albicans infections via increasing its apoptosis rate. Biochem. Biophys. Res. Commun. 451, 36–41.
Yeo I.K., Jang W.S., Min P.K., Cho S.W., Hong N.S., Kang J.S., et al. 2014. An epidemiological study of androgenic alopecia in 3114 Korean patients. Clin.Exp. Dermatol. 39(1): 25–29.
Yeo J. and Shahidi F. 2017. Effect of hydrothermal processing on changes of insoluble-bound phenoilcs of lentils. Journal of Functional Foods 38: 716–722.
Yimyam S., Suppansan P. and Jankajonchai K. 2023. Effectiveness of banana flower beverage on breast milk production among mothers of preterm neonates. J. Neonatal. Nurs. In press.
Zafer E. and Filiz I. 2009. Optimization of Drying of Olive Leaves in a Pilot-Scale Heat Pump Dryer. Dry. Technol. 27: 416–427.
Zhang J.A., Yin Z., Ma L.W., Yin Z.Q., Hu Y.Y., Xu Y., et al. 2014. The protective effect of baicalin against UVB irradiation induced photoaging: an in vitro and In vivo study. PLoS One 9: 1–13.
Zhang J., Kang L., Liu L.L., Wang D.D., Xu Y., Sheng S., et al. 2018. Caffeic acid as a preservative that extends shelf-life and maintains fruit quality of mulberries during cold storage. Afr. J. Agric. Res. 13(43): 2414–2422.