Biodecontamination of milk and dairy products by probiotics Boon for bane

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Razieh Sadat Mirmahdi
Alaleh Zoghi
Fatemeh Mohammadi
Kianoush Khosravi-Darani
Shima Jazaiery
Reza Mohammadi
Yasir Rehman


aflatoxins; biosorption; decontamination; heavy metals; dairy products


In recent decades, “contamination of the environment, food, and feed by different contaminants such as heavy metals and toxins is increasing due to industrial life.” Commercial milk and milk products can be contaminated with heavy metals and mycotoxins. Biosorption is a low-cost method and has good potential for decontamination. In dairy products, “various starters, especially probiotics, can be used as biosorbants, while microorganisms are able to bind to heavy metals and toxins and decrease their bioavailability and hazards in the human body.” In this article, the key role of dairy starters and probiotics in the decontamination of toxins and heavy metals, and the best probiotics for decontamination of aflatoxins and heavy metals has been reviewed. After a quick glance at introducing dairy products and the main risks in association with the intake of some hazardous materials from dairy products, the application of biological systems is mentioned. Then, the article is focused on the role of beneficial microorganisms as the last chance to decrease the risk of exposure to toxins and heavy metals in dairy products. This review can be helpful for biotechnologists and scientists who have challenges about the existence of heavy metals and toxins in milk and dairy products, and help them to find the best method to decrease the content of the usual contaminants.

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Abdelmotilib, N.M., Hamad, G.M., Elderea, H.B., Salem, E.G. and El Sohaimy, S.A., 2018. Aflatoxin M1 reduction in milk by a novel combination of probiotic bacterial and yeast strains. European Journal of Nutrition & Food Safety ,8(2),83–99.
Abedi, A.-S., Nasseri, E., Esfarjani, F., Mohammadi-Nasrabadi, F., Moosavi, M.H. and Hoseini, H., 2020. A systematic review and meta-analysis of lead and cadmium concentrations in cow milk in Iran and human health risk assessment. Environmental Science and Pollution Research 27: 10147–10159.
Adibpour, N., Soleimanian-Zad, S., Sarabi-Jamab, M. and Tajalli, F., 2016. Effect of storage time and concentration of aflatoxin m1 on toxin binding capacity of L. acidophilus in fermented milk product. Journal of Agricultural Science and Technology 18: 1209–1220.
Alani, M.S. and Al-Azzawi, M.N., 2015. Assessment of lead, cadmium and copper concentrations in raw milk collected from different location in Iraq. Iraqi Journal of Science 56: 350–355.
Alkorta, I., Hernández-Allica, J., Becerril, J., Amezaga, I., Albizu, I. and Garbisu, C., 2004. Recent findings on the phytoremediation of soils contaminated with environmentally toxic heavy metals and metalloids such as zinc, cadmium, lead, and arsenic. Reviews in Environmental Science and Biotechnology 3: 71–90.
Ansari, F., Khodaiyan, F., Rezaei, K. and Rahmani, A., 2015. Modelling of aflatoxin G1 reduction by kefir grain using response surface methodology. Journal of Environmental Health Science and Engineering 13: 40.
Asati, A., Pichhode, M. and Nikhil, K., 2016. Effect of heavy metals on plants: an overview. International Journal of Application or Innovation in Engineering & Management 5: 56–66.
Aslam, N., Tipu, M.Y., Ishaq, M., Cowling, A., McGill, D., Warriach, H.M. and Wynn, P., 2016. Higher levels of aflatoxin M1 contamination and poorer composition of milk supplied by informal milk marketing chains in Pakistan. Toxins 8: 347.
Bervis, N., Lorán, S., Juan, T., Carramiñana, J.J., Herrera, A., Ariño, A. and Herrera, M., 2021. Field monitoring of aflatoxins in feed and milk of high-yielding dairy cows under two feeding systems. Toxins 13: 201.
Bilandži?, N., Boži?, ?., ?oki?, M., Sedak, M., Kolanovi?, B.S., Varenina, I. and Cvetni?, Ž., 2014. Assessment of aflatoxin M1 contamination in the milk of four dairy species in Croatia. Food Control 43: 18–21.
Bilandži?, N., Tankovi?, S., Jeluši?, V., Varenina, I., Kolanovi?, B.S., Luburi?, ?.B. and Cvetni?, Ž., 2016. Aflatoxin M1 in raw and UHT cow milk collected in Bosnia and Herzegovina and Croatia. Food Control 68: 352–357.
Bokulich, N.A., Amiranashvili, L., Chitchyan, K., Ghazanchyan, N., Darbinyan, K., Gagelidze, N., Sadunishvili, T., Goginyan, V., Kvesitadze, G. and Torok, T., 2015. Microbial biogeography of the transnational fermented milk matsoni. Food Microbiology 50: 12–19.
Bovo, F., Franco, L.T., Rosim, R.E. and Oliveira, C.A.F.D., 2014. Ability of a Lactobacillus rhamnosus strain cultured in milk whey based medium to bind aflatoxin B1. Food Science and Technology 34: 566–570.
Camaj, A., Meyer, K., Berisha, B., Arbneshi, T. and Haziri, A., 2018. Aflatoxin M 1 contamination of raw cow’s milk in five regions of Kosovo during 2016. Mycotoxin Research 34: 205–209.
Campagnollo, F.B., Khaneghah, A.M., Borges, L.L., Bonato, M.A., Fakhri, Y., Barbalho, C.B., Barbalho, R.L., Corassin, C.H. and Oliveira, C.A., 2020. In vitro and in vivo capacity of yeast-based products to bind to aflatoxins B1 and M1 in media and foodstuffs: a systematic review and meta-analysis. Food Research International 137: 109505.
Castro-González, N.P., Calderón-Sánchez, F., Castro de Jesús, J., Moreno-Rojas, R., Tamariz-Flores, J.V., Pérez-Sato, M. and Soní-Guillermo, E., 2018. Heavy metals in cow’s milk and cheese produced in areas irrigated with waste water in Puebla, Mexico. Food Additives & Contaminants: Part B 11: 33–36.
Cherni, Y., Botta, C., Kasmi, M., Franciosa, I., Cocolin, L., Chatti, A., Trabelsi, I. and Elleuch, L., 2020. Mixed culture of Lactococcus lactis and Kluyveromyces marxianus isolated from kefir grains for pollutants load removal from Jebel Chakir Leachate. Water Environment Research, 92, 2041-2048.
Commission, E., 2006. Commission Regulation (EC) No 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. Official Journal of the European Union 364: 5–24.
Corassin, C., Bovo, F., Rosim, R. and Oliveira, C., 2013. Efficiency of Saccharomyces cerevisiae and lactic acid bacteria strains to bind aflatoxin M1 in UHT skim milk. Food Control 31: 80–83.
de Oliveira, T.M., Peres, J.A., Felsner, M.L. and Justi, K.C., 2017. Direct determination of Pb in raw milk by graphite furnace atomic absorption spectrometry (GF AAS) with electrothermal atomization sampling from slurries. Food Chemistry 229: 721–725.
De Roma, A., Rossini, C., Ritieni, A., Gallo, P. and Esposito, M., 2017. A survey on the Aflatoxin M1 occurrence and seasonal variation in buffalo and cow milk from Southern Italy. Food Control 81: 30–33.
Devegowda, G., Aravind, B. and Morton, M., 1996. Saccharomyces cerevisiae and mannanoligosaccharides to counteract aflatoxicosis in broilers. Proceedings of Australian Poultry Science Symposium, Sydney. pp. 106.
Dimitrieska-Stojkovi?, E., Stojanovska-Dimzoska, B., Ilievska, G., Uzunov, R., Stojkovi?, G., Hajrulai-Musliu, Z. and Jankuloski, D., 2016. Assessment of aflatoxin contamination in raw milk and feed in Macedonia during 2013. Food Control 59: 201–206.
Dyomina, M., Goroz, Y.O., Kalyuzhnaya, O. and Strelnikov, L., 2017. Technology of preparation of homemade fermented milk products and study their microflora. National University of Pharmacy, Kharkiv, Ukraine
Eker, F.Y., Muratoglu, K. and Eser, A.G., 2019. Detection of aflatoxin M 1 in milk and milk products in Turkey. Environmental Monitoring and Assessment 191: 1–8.
El Khoury, A., Atoui, A. and Yaghi, J., 2011. Analysis of aflatoxin M1 in milk and yogurt and AFM1 reduction by lactic acid bacteria used in Lebanese industry. Food Control 22: 1695–1699.
El-Nezami, H., Polychronaki, N., Salminen, S. and Mykkänen, H., 2002. Binding rather than metabolism may explain the interaction of two food-grade Lactobacillus strains with zearalenone and its derivative ??-Zearalenol. Applied and Environmental Microbiology 68: 3545–3549.
El Sheikha, A., 2015. Food safety issues in Saudi Arabia. Nutrition and Food Technology 1: 1–4.
El Sheikha, A.F., 2019. Molecular detection of mycotoxigenic fungi in foods: the case for using PCR-DGGE. Food Biotechnology 33: 54–108.
El Sheikha, A.F., Levin, R.E. and Xu, J., 2018. Molecular techniques in food biology: safety, biotechnology, authenticity and traceability. John Wiley & Sons.
El Sheikha, A.F. and Montet, D., 2014. African fermented foods: historical roots and real benefits. Microorganisms and fermentation of traditional foods. CRC Press, Taylor and Francis Group, Boca Raton, FL, pp. 248–282.
Elsanhoty, R.M., Salam, S.A., Ramadan, M.F. and Badr, F.H., 2014. Detoxification of aflatoxin M1 in yoghurt using probiotics and lactic acid bacteria. Food Control 43: 129–134.
Fallah, A.A., Barani, A. and Nasiri, Z., 2015. Aflatoxin M1 in raw milk in Qazvin Province, Iran: a seasonal study. Food Additives & Contaminants: Part B 8: 195–198.
FAO/WHO, 2001. Evaluation of health and nutritional properties of powder milk and live lactic acid bacteria, pp. 1-4.
Fazeli, M.R., Hajimohammadali, M., Moshkani, A., Samadi, N., Jamalifar, H., Khoshayand, M.R., Vaghari, E. and Pouragahi, S., 2009. Aflatoxin B1 binding capacity of autochthonous strains of lactic acid bacteria. Journal of Food Protection 72: 189–192.
Ferreira, A. and Viljoen, B., 2003. Yeasts as adjunct starters in matured Cheddar cheese. International Journal of Food Microbiology 86: 131–140.
Fguiri, I., Ziadi, M., Abassi, M., Arroum, S. and Khorchani, T., 2013. Suitability of camel milk to transformation in Leben by lactic starter. African Journal of Microbiology Research 6: 7185–7192.
Foroughi, M., Sarabi Jamab, M., Keramat, J. and Foroughi, M., 2018. Immobilization of Saccharomyces cerevisiae on Perlite Beads for the Decontamination of Aflatoxin M1 in Milk. Journal of Food Science 83: 2008–2013.
Garrote, G.L., Abraham, A.G. and De Antoni, G.L., 2001. Chemical and microbiological characterisation of kefir grains. The Journal of Dairy Research 68: 639.
Giovati, L., Magliani, W., Ciociola, T., Santinoli, C., Conti, S. and Polonelli, L., 2015. AFM1 in milk: physical, biological, and prophylactic methods to mitigate contamination. Toxins 7: 4330–4349.
Göksungur, Y., Üren, S. and Güvenç, U., 2005. Biosorption of cadmium and lead ions by ethanol treated waste baker’s yeast biomass. Bioresource Technology 96: 103–109.
GONÇALVES, Bruna Leonel et al. Aflatoxin M1: biological decontamination methods in milk and cheese. Food Science and Technology [online]. 2020 [Accessed 18 June 2021] , Available from: . Epub 09 Oct 2020. ISSN 1678-457X.
Granados-Chinchilla, Fabio., 2016. Insights into the interaction of milk and dairy proteins with aflatoxin m1. In: Isabel, G. (ed.) Milk proteins—from structure to biological properties and health aspects, pp. 265–286.
Gupta, R., Bimlesh, M., Joshi, V. and Prasad, D., 2000. Microbiological, chemical and ultrastructural characteristics of Mishti Doi (Sweetened Dahi). Journal of Food Science and Technology (Mysore) 37: 54–57.
Hadiani, M.R., Darani, K.K., Rahimifard, N. and Younesi, H., 2018. Biosorption of low concentration levels of Lead (II) and Cadmium (II) from aqueous solution by Saccharomyces cerevisiae: response surface methodology. Biocatalysis and Agricultural Biotechnology 15: 25–34.
Hadiani, M.R., Khosravi-Darani, K. and Rahimifard, N., 2019. Optimization of As (III) and As (V) removal by Saccharomyces cerevisiae biomass for biosorption of critical levels in the food and water resources. Journal of Environmental Chemical Engineering 7: 102949.
Halagarda, M., Ptasinska-Marcinkiewicz, J. and Fijorek, K., 2018. A comparison of mineral elements content in conventional and organic milk from Southern Poland. ?ywno?? Nauka Technologia Jako?? 25: 137-150.
Hashim, M. and Chu, K., 2004. Biosorption of cadmium by brown, green, and red seaweeds. Chemical Engineering Journal 97: 249–255.
Haskard, C., Binnion, C. and Ahokas, J., 2000. Factors affecting the sequestration of aflatoxin by Lactobacillusrhamnosus strain GG. Chemico-Biological Interactions 128: 39–49.
Haskard, C.A., El-Nezami, H.S., Kankaanpää, P.E., Salminen, S. and Ahokas, J.T., 2001. Surface binding of aflatoxin B1 by lactic acid bacteria. Applied and Environmental Microbiology 67: 3086–3091.
Hayat, K., Menhas, S., Bundschuh, J. and Chaudhary, H.J., 2017. Microbial biotechnology as an emerging industrial wastewater treatment process for arsenic mitigation: a critical review. Journal of Cleaner Production 151: 427–438.
Huang, L., Zheng, N., Zheng, B., Wen, F., Cheng, J., Han, R., Xu, X., Li, S. and Wang, J., 2014. Simultaneous determination of aflatoxin M1, ochratoxin A, zearalenone and ?-zearalenol in milk by UHPLC–MS/MS. Food Chemistry 146: 242–249.
Iha, M.H., Barbosa, C.B., Okada, I.A. and Trucksess, M.W., 2013. Aflatoxin M1 in milk and distribution and stability of aflatoxin M1 during production and storage of yoghurt and cheese. Food Control 29: 1–6.
Ismail, A., Levin, R.E., Riaz, M., Akhtar, S., Gong, Y.Y. and de Oliveira, C.A., 2017. Effect of different microbial concentrations on binding of aflatoxin M1 and stability testing. Food Control 73: 492–496.
Ismail, A., Riaz, M., Akhtar, S., Ismail, T., Ahmad, Z. and Hashmi, M.S., 2015. Estimated daily intake and health risk of heavy metals by consumption of milk. Food Additives & Contaminants: Part B 8: 260–265.
Javanbakht, V., Alavi, S.A. and Zilouei, H., 2014. Mechanisms of heavy metal removal using microorganisms as biosorbent. Water Science and Technology 69: 1775–1787.
Kamyar, S. and Movassaghghazani, M., 2017. Reduction of Aflatoxin M1 in milk using Kefir Starter. Iranian Journal of Toxicology 11: 27–31.
Karazhiyan, H., Mehraban, S.M., Karazhyan, R., Mehrzad, A. and Haghighi, E., 2016. Ability of different treatments of Saccharomyces cerevisiae to surface bind aflatoxin M1 in yoghurt. Journal of Agricultural science and Technology, 18:1489-1498.
Karczmarczyk, A., Baeumner, A.J. and Feller, K.-H., 2017. Rapid and sensitive inhibition-based assay for the electrochemical detection of Ochratoxin A and Aflatoxin M1 in red wine and milk. Electrochimica Acta 243: 82–89.
Khairul, I., Wang, Q.Q., Jiang, Y.H., Wang, C. and Naranmandura, H., 2017. Metabolism, toxicity and anticancer activities of arsenic compounds. Oncotarget 8: 23905.
Khosravi-Darani, K., Barzegar, F. and Baghdadi, M., 2019. Detoxification of heterocyclic aromatic amines by probiotic to inhibit medical hazards. Mini Reviews in Medicinal Chemistry 19: 1196–1203.
Kos, J., Levi?, J., ?uragi?, O., Koki?, B. and Miladinovi?, I., 2014. Occurrence and estimation of aflatoxin M1 exposure in milk in Serbia. Food Control 38: 41–46.
Kuboka, M.M., Imungi, J.K., Njue, L., Mutua, F., Grace, D. and Lindahl, J.F., 2019. Occurrence of aflatoxin M1 in raw milk traded in peri-urban Nairobi, and the effect of boiling and fermentation. Infection Ecology & Epidemiology 9: 1625703.
Kumar, R., Kaur, M., Garsa, A.K., Shrivastava, B., Reddy, V. and Tyagi, A., 2015. Natural and cultured buttermilk. In: Fermented milk and dairy products, pp. 203–225.
Lahtinen, S., Haskard, C., Ouwehand, A., Salminen, S. and Ahokas, J., 2004. Binding of aflatoxin B1 to cell wall components of Lactobacillus rhamnosus strain GG. Food Additives and Contaminants 21: 158–164.
Macori, G. and Cotter, P.D., 2018. Novel insights into the microbiology of fermented dairy foods. Current Opinion in Biotechnology 49: 172–178.
Malhat, F., Hagag, M. and Saber, A., 2012. Contamination of cows milk by heavy metal in Egypt. Bulletin of Environmental Contamination and Toxicology 88: 611–613.
Mannani, N., Tabarani, A., El Adlouni, C. and Zinedine, A., 2021. Aflatoxin M1 in pasteurized and UHT milk marked in Morocco. Food Control 124: 107893.
Masoud, R., Khosravi-Darani, K., Sharifan, A., Asadi, G.-H. and Younesi, H., 2020. The biosorption capacity of Saccharomyces cerevisiae for cadmium in milk.
Massoud, R., Cruz, A. and Darani, K.K., 2018. Ochratoxin A: from safety aspects to prevention and remediation strategies. Current Nutrition & Food Science 14: 11–16.
Massoud, R., Khosravi-Darani, K., Sharifan, A. and Asadi, G.H., 2019. Lead bioremoval from milk by Saccharomyces cerevisiae. Biocatalysis and Agricultural Biotechnology 22: 101437.
Massoud, R., Khosravi-Darani, K., Sharifan, A., Asadi, G.H. and Younesi, H., 2020a. The biosorption capacity of Saccharomyces cerevisiae for cadmium in milk. Dairy (journal) 1: 169–176.
Massoud, R., Khosravi?Darani, K., Sharifan, A., Asadi, G. and Zoghi, A., 2020b. Lead and cadmium biosorption from milk by Lactobacillus acidophilus ATCC 4356. Food Science & Nutrition 8: 5284–5291.
Massoud, R., Sharifan, A., Khosravi?Darani, K. and Asadi, G., 2021. Mercury biosorption process by using Saccharomyces cerevisiae in milk. Journal of Food Processing and Preservation 45: e15008.
Meshref, A.M., Moselhy, W.A. and Hassan, N.E.-H.Y., 2014. Heavy metals and trace elements levels in milk and milk products. Journal of Food Measurement and Characterization 8: 381–388.
Michlig, N., Signorini, M., Gaggiotti, M., Chiericatti, C., Basílico, J.C., Repetti, M.R. and Beldoménico, H.R., 2016. Risk factors associated with the presence of aflatoxin M1 in raw bulk milk from Argentina. Food Control 64: 151–156.
Milanowski, M., Pomastowski, P., Railean-Plugaru, V., Rafi?ska, K., Ligor, T. and Buszewski, B., 2017. Biosorption of silver cations onto Lactococcus lactis and Lactobacillus casei isolated from dairy products. PLoS One 12: e0174521.
Miura, N., Yoshioka, H., Ashimori, A., Ohtani, K., Hasegawa, T., Hwang, G.-W., Ikeda, M. and Nonogaki, T., 2017. Multidirectional analyses of hepatic chronotoxicity induced by cadmium in mice. The Journal of Toxicological Sciences 42: 597–604.
Motameny, R., Sadeghi, A., Dehghan-Banadaky, M., Chamani, M. and Abolhassani, M., 2012. Effect of some acid treated bacteria on reduction of impure aflatoxin B1 in ruminant gastrointestinal model. The Journal of American Science 8: 213–217.
Muhib, M.I., Chowdhury, M.A.Z., Easha, N.J., Rahman, M.M., Shammi, M., Fardous, Z., Bari, M.L., Uddin, M.K., Kurasaki, M. and Alam, M.K., 2016. Investigation of heavy metal contents in cow milk samples from area of Dhaka, Bangladesh. International Journal of Food Contamination 3: 1–10.
Naidoo, S.V.K., Bester, M.J., Arbi, S., Venter, C., Dhanraj, P. and Oberholzer, H.M., 2019. Oral exposure to cadmium and mercury alone and in combination causes damage to the lung tissue of Sprague-Dawley rats. Environmental Toxicology and Pharmacology 69: 86–94.
Njombwa, C.A., Moreira, V., Williams, C., Aryana, K. and Matumba, L., 2021. Aflatoxin M 1 in raw cow milk and associated hepatocellular carcinoma risk among dairy farming households in Malawi. Mycotoxin Research 37: 89–96.
Patterson,W James. and Minear, Roger., 2013. Physical-chemical methods of heavy metals removal. In: Heavy metals in the aquatic environment. Elsevier, Amsterdam, the Netherlands, pp. 261–276.
Peña-Rodas, O., Martinez-Lopez, R. and Hernandez-Rauda, R., 2018. Occurrence of Aflatoxin M1 in cow milk in El Salvador: results from a two-year survey. Toxicology Reports 5: 671–678.
Porova, N., Botvinnikova, V., Krasulya, O., Cherepanov, P. and Potoroko, I., 2014. Effect of ultrasonic treatment on heavy metal decontamination in milk. Ultrasonics Sonochemistry 21: 2107–2111.
Poulsen, P., 1970. Ymer’, a Danish protein-enriched fermented milk-product. XVIII International Dairy Congress, Sydney.
Puga-Torres, B., Salazar, D., Cachiguango, M., Cisneros, G. and Gómez-Bravo, C., 2020. Determination of aflatoxin M1 in raw milk from different provinces of Ecuador. Toxins 12: 498.
Raftaniamiri, Z., Khandelwal, P. and Aruna, B.R., 2010. Development of acidophilus milk via selected probiotics & prebiotics using artificial neural network. Advances in Bioscience and Biotechnology Vol.01No.03: 4. 10.4236/abb.2010.13031

Ray,C Ramesh., El Sheikha, Aly .F. and Kumar, Sasi., 2014. Oriental fermented functional (probiotic) foods. In: Microorganisms and fermentation of traditional foods. Food biology series. Science Publishers Inc., Boca Raton, FL, pp. 283–311.
Redzwan, S.M., Abd Mutalib, M.S., Wang, J.-S., Ahmad, Z., Kang, M.-S., Nasrabadi, E.N. and Jamaluddin, R., 2016. Effect of supplementation of fermented milk drink containing probiotic Lactobacillus casei Shirota on the concentrations of aflatoxin biomarkers among employees of Universiti Putra Malaysia: a randomised, double-blind, cross-over, placebo-controlled study. British Journal of Nutrition 115: 39–54.
Rodríguez-Blanco, M., Ramos, A., Prim, M., Sanchis, V. and Marín, S., 2020. Usefulness of the analytical control of aflatoxins in feedstuffs for dairy cows for the prevention of aflatoxin M 1 in milk. Mycotoxin Research 36: 11–22.
Ryan, P., Ross, R., Fitzgerald, G., Caplice, N. and Stanton, C., 2015. Sugar-coated: exopolysaccharide producing lactic acid bacteria for food and human health applications. Food & Function 6: 679–693.
Salque, M., Bogucki, P.I., Pyzel, J., Sobkowiak-Tabaka, I., Grygiel, R., Szmyt, M. and Evershed, R.P., 2013. Earliest evidence for cheese making in the sixth millennium BC in northern Europe. Nature 493: 522–525.
Sanders, M.E., Lenoir?Wijnkoop, I., Salminen, S., Merenstein, D.J., Gibson, G.R., Petschow, B.W., Nieuwdorp, M., Tancredi, D.J., Cifelli, C.J. and Jacques, P., 2014. Probiotics and prebiotics: prospects for public health and nutritional recommendations. Annals of the New York Academy of Sciences 1309: 19–29.
Sani, A.M., Marhamati, Z. and Marhamatizade, M., 2014. Bio-detoxification of aflatoxin M1 in kefir using Lactobacillus casei. Biotechnology: An Indian Journal 9: 219–224.
Sarimehmeto?lu, B. and Küplülü, Ö., 2004. Binding ability of aflatoxin M1 to yoghurt bacteria. Ankara Üniv Vet Fak Derg, 51: 195-198.
Sarlak, Z., Rouhi, M., Mohammadi, R., Khaksar, R., Mortazavian, A.M., Sohrabvandi, S. and Garavand, F., 2017. Probiotic biological strategies to decontaminate aflatoxin M1 in a traditional Iranian fermented milk drink (Doogh). Food Control 71: 152–159.
Sarlak, Z.A.K.K.-D., 2020. Probiotics: the last trench for environmental pollution of organophosphorus pesticides. Journal of Health Sciences and Enviornments. submitted, revised.
Satyapal, G., Rani, S., Kumar, M. and Kumar, N., 2016. Potential role of arsenic resistant bacteria in bioremediation: current status and future prospects. Journal of Microbial and Biochemical Technology 8: 256–258.
Se?meno?lu, M.S. and Baydan, E., 2021. Comparison of heavy metal levels of organic and conventional milk and milk products in Turkey. Turkish Journal of Agriculture-Food Science and Technology 9: 696–700.
Sevim, S., Topal, G.G., Tengilimoglu-Metin, M.M., Sancak, B. and Kizil, M., 2019. Effects of inulin and lactic acid bacteria strains on aflatoxin M1 detoxification in yoghurt. Food Control 100: 235–239.
Shahbazi, Y., Ahmadi, F. and Fakhari, F., 2016. Voltammetric determination of Pb, Cd, Zn, Cu and Se in milk and dairy products collected from Iran: an emphasis on permissible limits and risk assessment of exposure to heavy metals. Food Chemistry 192: 1060–1067.
Sharma, H., Jadhav, V.J. and Garg, S.R., 2020. Aflatoxin M1 in milk in Hisar city, Haryana, India and risk assessment. Food Additives & Contaminants: Part B 13: 59–63.
Shetty, P.H. and Jespersen, L., 2006. Saccharomyces cerevisiae and lactic acid bacteria as potential mycotoxin decontaminating agents. Trends in Food Science & Technology 17: 48–55.
Shigute, T. and Washe, A.P., 2018. Reduction of aflatoxin M1 levels during ethiopian traditional fermented milk (Ergo) production. Journal of Food Quality 2018, 10 pages.
Shuib, N.S., Makahleh, A., Salhimi, S.M. and Saad, B., 2017. Natural occurrence of aflatoxin M1 in fresh cow milk and human milk in Penang, Malaysia. Food Control 73: 966–970.
Simova, E., Beshkova, D., Angelov, A., Hristozova, T., Frengova, G. and Spasov, Z., 2002. Lactic acid bacteria and yeasts in kefir grains and kefir made from them. Journal of Industrial Microbiology and Biotechnology 28: 1–6.
Sulaymon, A.H., Mohammed, A.A. and Al-Musawi, T.J., 2013. Competitive biosorption of lead, cadmium, copper, and arsenic ions using algae. Environmental Science and Pollution Research 20: 3011–3023.
Suturovi?, Z., Kravi?, S., Milanovi?, S., ?urovi?, A. and Brezo, T., 2014. Determination of heavy metals in milk and fermented milk products by potentiometric stripping analysis with constant inverse current in the analytical step. Food Chemistry 155: 120–125.
Taheur, F.B., Fedhila, K., Chaieb, K., Kouidhi, B., Bakhrouf, A. and Abrunhosa, L., 2017. Adsorption of aflatoxin B1, zearalenone and ochratoxin A by microorganisms isolated from Kefir grains. International Journal of Food Microbiology 251: 1–7.
Tamime, A., Muir, D., Khaskheli, M. and Barclay, M., 2000. Effect of processing conditions and raw materials on the properties of Kishk 1. Compositional and microbiological qualities. LWT-Food Science and Technology 33: 444–451.
Turbic, A., Ahokas, J. and Haskard, C., 2002. Selective in vitro binding of dietary mutagens, individually or in combination, by lactic acid bacteria. Food Additives & Contaminants 19: 144–152.
U.S. Food and Drug Administration, 2019. Levels for aflatoxins in animal food, compliance policy guide. Available at: (accessed on 16 July 2019).
Venâncio, R.L., Ludovico, A., de Santana, E.H.W., de Toledo, E.A., de Almeida Rego, F.C. and Dos Santos, J.S., 2019. Occurrence and seasonality of aflatoxin M1 in milk in two different climate zones. Journal of the Science of Food and Agriculture 99: 3203–3206.
Vishnoi, N., Dixit, S. and Singh, D., 2014. Surface binding and intracellular uptake of arsenic in bacteria isolated from arsenic contaminated site. Ecological Engineering 73: 569–578.
Wochner, K.F., Becker-Algeri, T.A., Colla, E., Badiale-Furlong, E. and Drunkler, D.A., 2018. The action of probiotic microorganisms on chemical contaminants in milk. Critical Reviews in Microbiology 44: 112–123.
Yadav, H., Jain, S. and Sinha, P., 2005. Preparation of low fat probiotic dahi. Journal of Dairying, Foods and Home Sciences 24: 172–177.
Yang, Q., Li, Z., Lu, X., Duan, Q., Huang, L. and Bi, J., 2018. A review of soil heavy metal pollution from industrial and agricultural regions in China: pollution and risk assessment. Science of the Total Environment 642: 690–700.
Yerlikaya, O., 2014. Starter cultures used in probiotic dairy product preparation and popular probiotic dairy drinks. Food Science and Technology 34: 221–229.
Yiannikouris, A., André, G., Buléon, A., Jeminet, G., Canet, I., François, J., Bertin, G. and Jouany, J.-P., 2004. Comprehensive conformational study of key interactions involved in zearalenone complexation with ?-D-glucans. Biomacromolecules 5: 2176–2185.
Yu, B., Chang, C.-H. and Lee, T.-T., 2015. Effects of the probiotics supplementation in diet on intestinal microflora ecosystem in broilers. Journal of Advanced Agricultural Technologies ,2: 138-142 .
Zakaria, A.M., Amin, Y.A., Khalil, O.S.F., Abdelhiee, E.Y. and Elkamshishi, M.M., 2019. Rapid detection of aflatoxin M1 residues in market milk in Aswan Province, Egypt and effect of probiotics on its residues concentration. Journal of Advanced Veterinary and Animal Research 6: 197.
Zeynab, R., Pratima, K. and Aruna, B.R., 2010. Development of acidophilus milk via selected probiotics & prebiotics using artificial neural network. Advances in Bioscience and Biotechnology , 1, 224-231. doi: 10.4236/abb.2010.13031.
Zhang, W.-L., Du, Y., Zhai, M.-M. and Shang, Q., 2014. Cadmium exposure and its health effects: a 19-year follow-up study of a polluted area in China. Science of the Total Environment 470: 224–228.
Ziarati, P., Shirkhan, F., Mostafidi, M. and Zahedi, M.T., 2018. An overview of the heavy metal contamination in milk and dairy products. Acta Scientific Pharmaceutical Sciences 2: 1–14.
Zoghi, A., Khosravi-Darani, K. and Sohrabvandi, S., 2014. Surface binding of toxins and heavy metals by probiotics. Mini Reviews in Medicinal Chemistry 14: 84–98.
Zoghi, A., Khosravi?Darani, K., Sohrabvandi, S. and Attar, H., 2019. Patulin removal from synbiotic apple juice using Lactobacillus plantarum ATCC 8014. Journal of Applied Microbiology 126: 1149–1160.
Zoghi, A., Khosravi?Darani, K., Sohrabvandi, S., Attar, H. and Alavi, S.A., 2017. Effect of probiotics on patulin removal from synbiotic apple juice. Journal of the Science of Food and Agriculture 97: 2601–2609.
Zoghi, A., Khosravi Darani, K. and Hekmatdoost, A., 2020. Effects of pretreatments on patulin removal from apple juices using Lactobacilli: binding stability in simulated gastrointestinal condition and modeling. Probiotics and Antimicrobial Proteins 1–11.