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Carrot is being the most comestible root vegetable due its important source of nutritional compounds mainly carotenoids, sugars and phenols. In Tunisia, despite the genetic diversity observed in carrot germplasm including landraces and wild relatives, no researches had been conducted on biochemical composition of carrot. Thus, this study aims to analyze carotenoids, soluble sugars, total phenols, total flavonoids and colour proprieties of fourteen carrot landraces, in order to determine the diversity among them and to analyze the relationships among their biochemical contents. Carotenoids and soluble sugars were analyzed by HPLC, and total phenols and total flavonoids by spectrophotometer. The main carotenoids identified were ?-carotene, ?-carotene and lutein, and the major sugars were sucrose, glucose, fructose and galactose. Significant differences were observed between Tunisian carrot landraces with respect to their biochemical composition and colour characteristics. Total carotenoids and total sugars ranged from 155.74 to 511.44 ?g/g of dw and from 368.77 to 546.79 mg/g of dw, respectively. Total phenols and total flavonoids varied from 24.13 to 41.39 mgGAE/100g of dw and from 16.51 to 24.85 µgCE/100 g of dw, respectively. Significant, positive and negative correlations were found between the measured parameters. A principal component analysis (PCA) and agglomerative hierarchical clustering (AHC) were performed to classify Tunisian carrot landraces on the base of colour properties and phytochemical compounds. PCA divided the studied landraces into four main groups and AHC classified them into three different clusters. Tunisian carrot landraces were found to be rich on bioactive antioxidant compounds, they could be good candidates for future breeding programs.