PREBIOTIC EFFECTS OF XYLANASE MODIFICATION OF ?-GLUCAN FROM OAT BRAN ON BIFIDOBACTERIUM BIFIDUM

Main Article Content

X.J. QIU
W.X. ZHENG
L. ZHANG
Y.L. SHI
J.H. HU
Y.L. LI
Z.Y. LIU
M.D. ZHU

Keywords

β-glucan, Bifidobacterium bifidum, modification, prebiotic, xylanase

Abstract

Oat ?-glucan (BG) was isolated from oat bran, and the xylanase treatment was conducted to obtain modification of ?-glucan (MBG). The relative molecular weight  (Mw) of BG and MBG was determined by gel permeation chromatography  (GPC). Results demonstrated that the Mw of BG was reduced from 1.66×104 to 5.43×103. We assessed the prebiotic effect of BG and MBG on human colon Bifidobacterium bifidum?B. bifidum). Our findings suggest that the addition of BG and MBG resulted in a lower pH of the fermentation broths. Both lactic and acetic acid production increased in the fermentation broths. While BG was found to significantly promote the proliferation of B. bifidum, MBG had a greater effect on B. bifidum.

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References

Berecochea-Lopez A., Decorde K., Ventura E., Godard M., Bornet A., Teissedre P.L., Cristol J.P. and Rouanet JM. 2009. Fungal chitin-glucan from Aspergillusnigerefficiently reduces aortic fatty streak accumulation in the high-fat fed hamster, an animal model of nutritionally induced atherosclerosis. J. Agr. Food Chem. 57:1093-1098.

Gibson G.R., Hutkins R., Sanders M.E., Prescott S.L., Reimer R.A., Salminen S.J. and Reid G. 2017. The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics. Nat Rev Gastro Hepat, 14:491-502.

Holscher H.D., Caporaso J.G., Hooda S., Brulc J.M., Fahey G.C. and Swanson K.S. 2015. Fiber supplementation influences phylogenetic structure and functional capacity of the human intestinal microbiome: follow-up of a randomized controlled trial. Am. J. Clin. Nutr. 101:55-64.

Laurikainen T., Harkonen H., Autio K. and Poutanen K. 1998. Effects of enzymes in fibre-enriched baking. J Sci. Food Agric. 76:239-249.

Lebesi D.M. and Tzia C. 2012. Use of endoxylanase treated cereal brans for development of dietary fiber enriched cakes. Innov.Food Sci. Emerg. 13:207-214.

Lecerf J.M., Dépeint F., Clerc E., Dugenet Y., Niamba C.N. and Rhazi L. 2012. Xylo-oligosaccharide (XOS) in combination with inulin modulates both the intestinal environment and immune status in healthy subjects, while XOS alone only shows prebiotic properties. Brit. J. Nutr.108:1847-1858.

Louis P., Scott K.P., Duncan S.H. and Flint H.J. 2007. Understanding the effects of diet on bacterial metabolism in the large intestine. J. Appl. Microbiol. 102:1197-1208.

Li Y., Fan Y.H., Pan H.O., Qian H.F., Qi X.G., Wu G.C., Zhang H., Xu M.J. and Wang L. 2018. Effects of functional beta-glucan on proliferation, differentiation, metabolism and its anti-fibrosis properties in muscle cells. Int. J. Biol. Macromol. 117:287-293.

Liu L., Wen W., Zhang RF., Wei ZC., Deng YY., Xiao J. and Zhang M.W. 2017. Complex enzyme hydrolysis releases antioxidative phenolics from rice bran. Food Chem. 214:1-8.

Ma M. and Mu T. 2016. Modification of deoiled cumin dietary fiber with laccase and cellulase under high hydrostatic pressure. Carbohyd Polym. 136:87-94.

Mei K.L., Tung Y.L. and Chia C.C. 2018. Chemical identification of a sulfated glucan from Antrodia cinnamomea and its anti-cancer functions via inhibition of EGFR and mTOR activity. Carbohyd Polym. 202:536-544.

Neyrinck A.M., Possemiers S., Verstraete W., De Backer F., Cani P.D. and Delzenne N.M. 2012. Dietary modulation of clostridial cluster XIVa gut bacteria (Roseburia spp.) by chitin-glucan fiber improves host metabolic alterations induced by high-fat diet in mice. J. Nutr. Biochem. 23:51-59.

Pins J.J., Keenan J.M., Curry L.L., Goulson M.J. and Kolberg L.W. 2005. Extracted barley beta-glucan improves CVD risk factors and other biomarkers in a population of generally healthy hypercholesterolemic men and women. Prevention and Control 1:131-136.

Santala O., Kiran A., Sozer N., Poutanen K. and Nordlund E. 2014. Enzymatic modification and particle size reduction of wheat bran improves the mechanical properties and structure of bran-enriched expanded extrudates. J. Cereal Sci. 60:448-456.

Saulnier L., Robert P., Grintchenko M., Jamme F., Bouchet B. and Guillon F. 2009. Wheat endosperm cell walls: Spatial heterogeneity of polysaccharide structure and composition using micro-scale enzymatic fingerprinting and FT-IR microspectroscopy. J. Cereal Sci. 50:312-317.

Slavin J. 2013. Fiber and prebiotics: mechanisms and health benefits. Nutrients 5:1417-1435.

Tan B. and Ren B. 2006. Current status and trends of research and development of deep processing technology for coarse grain resources. J. Chin. Cereal. 21:229-235.

Tap J., Furet J.P., Bensaada M., Philippe C., Roth H., Rabot S., Lakhdari O., Lombard V., Henrissat B., Corthier G., Fontaine E., Dore J. and Leclerc M. 2015. Gut microbiota richness promotes its stability upon increased dietary fibre intake in healthy adults. Environ.Microbiol. 17:4954-4964.

Verbeke K.A., Boobis A.R., Chiodini A., Edwards C.A., Franck A., Kleerebezem M., Nauta A., Raes J. and Tuohy K.M. 2015. Towards microbial fermentation metabolites as markers for health benefits of prebiotics. Nutr. Res. Rev. 28:42-66.

Vinolo M.A., Rodrigues H.G., Nachbar R.T. and Curi R. 2011.Regulation of inflammation by short chain fatty acids. Nutrients 3:858-876.

Vitaglione P., Napolitano A. and Fogliano V. 2008.Cereal dietary fibre: a natural functional ingredient to deliver phenolic compounds into the gut. Trends Food Sci.Tech. 19:451-463.

Wilson T.A., Nicolosi R.J., Delaney B., Chadwell K. and Moolchandani V. 2004 Reduced and high molecular weight barley beta-glucans decrease plasma total and non-HDL cholesterol in hypercholesterolemic Syrian golden hamsters. J. Nutr.134, 2617-262.

Wong J.M., de Souza R., Kendall C.W., Emam A. and Jenkins D.J.A. 2006. Colonic health: fermentation and short chain fatty acids. J. Clin. Gastroenterol. 40:235-243.

Wen Y., Niu M., Zhang B.J., Zhao SM. and Xiong S.B. 2017. Structural characteristics and functional properties of rice bran dietary fiber modified by enzymatic and enzyme-micronization treatments. LWT -Food Sci. Technol. 75:344-351.

Cao Y., Sun Y., Zou S.W., Li M.X. and Xu XJ. 2017. Orally administered baker's yeast beta-Glucan promotes glucose and lipid homeostasis in the livers of obesity and diabetes model mice. J. Agr. Food Chem. 65:9665-9674.

Zheng W., Liu Z. and Shi Y. 2017. Research progress on preparation of soluble dietary fiber in food processing by-products. Food and Feed Industry. 12:31-35.